Exploration of essential gene functions via titratable promoter alleles
| Autor: | Jason Moffat, Chris A. Kaiser, Charles Boone, Christopher J. Roberts, Nevan J. Krogan, Miles Trochesset, Xueqi Yang, Gordon Chua, Jennifer Haynes, Andres Lopez, Nicholas Mitsakakis, Shawna L. Hiley, Wen-Tao Peng, Armaity P. Davierwala, Brenda J. Andrews, Jörg Grigull, Jeff Pootoolal, Darcy L Morse, Timothy P. Hughes, Jack Greenblatt, Sanie Mnaimneh, Wen Zhang, Zhijian Li, Quaid Morris |
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| Rok vydání: | 2004 |
| Předmět: |
Ribosomal Proteins
Saccharomyces cerevisiae Proteins Transcription Genetic Genes Fungal Ribosome biogenesis Saccharomyces cerevisiae Biology Genetic analysis General Biochemistry Genetics and Molecular Biology RNA Transfer Gene Expression Regulation Fungal Promoter Regions Genetic Gene Gene knockout Alleles Oligonucleotide Array Sequence Analysis Regulation of gene expression Genetics Feedback Physiological Genes Essential Models Genetic Biochemistry Genetics and Molecular Biology(all) Gene Expression Profiling Synthetic genetic array Mitochondria Gene expression profiling Pharmaceutical Preparations Essential gene Protein Processing Post-Translational Gene Deletion |
| Zdroj: | Cell. 118(1) |
| ISSN: | 0092-8674 |
| Popis: | Nearly 20% of yeast genes are required for viability, hindering genetic analysis with knockouts. We created promoter-shutoff strains for over two-thirds of all essential yeast genes and subjected them to morphological analysis, size profiling, drug sensitivity screening, and microarray expression profiling. We then used this compendium of data to ask which phenotypic features characterized different functional classes and used these to infer potential functions for uncharacterized genes. We identified genes involved in ribosome biogenesis (HAS1, URB1, and URB2), protein secretion (SEC39), mitochondrial import (MIM1), and tRNA charging (GSN1). In addition, apparent negative feedback transcriptional regulation of both ribosome biogenesis and the proteasome was observed. We furthermore show that these strains are compatible with automated genetic analysis. This study underscores the importance of analyzing mutant phenotypes and provides a resource to complement the yeast knockout collection. |
| Databáze: | OpenAIRE |
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