In vivo operation of the pentose phosphate pathway in frog oocytes is limited by NADP+availability
Autor: | Victoria Guixé, Ana Preller, Tito Ureta |
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Rok vydání: | 1999 |
Předmět: |
Biophysics
Pentose Stimulation Biology Pentose phosphate pathway Carbohydrate metabolism Biochemistry Frog oocyte Substrate Specificity Pentose Phosphate Pathway Xenopus laevis Structural Biology In vivo Genetics Animals Pentose-phosphate pathway Molecular Biology chemistry.chemical_classification Glucose metabolism Rana pipiens Cell Biology Glucose chemistry NADP+ Oocytes Phenazine Methosulfate Female NAD+ kinase NADP Intracellular |
Zdroj: | FEBS Letters. 446:149-152 |
ISSN: | 0014-5793 |
Popis: | Evolution of CO2 from labelled glucose microinjected into frog oocytes in vivo may be ascribed to the pentose-P pathway, as measured by radioactive CO2 production from [1-14C] and [6-14C]glucose. Coinjection of NADP+ and [14C]glucose significantly stimulated 14CO2 production. The effect depends on the amount of NADP+ injected, half maximal stimulation being obtained at 0.13 mM. The increase in CO2 production was also observed with microinjected glucose-1-P, glucose-6-P or fructose-6-P used as substrates. Phenazine methosulfate, mimicked the effects of NADP+. A high NADPH/NADP+ ratio of 4.3 was found in the cells, the intracellular concentration of NADP+ being 19 μM. |
Databáze: | OpenAIRE |
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