Characterization of the influence of anti-hormone and/or anti-growth factor neutralizing antibodies on cell clone architecture and the growth of human neoplastic astrocytic cell lines
| Autor: | Sandrine Rorive, Robert Kiss, Thierry Gras, Isabelle Camby, Jean Lambert Pasteels, Anna Kruczynski, Isabelle Salmon, André Danguy, Francis Darro |
|---|---|
| Rok vydání: | 1994 |
| Předmět: |
Cancer Research
medicine.medical_specialty medicine.medical_treatment Cell Biology Gonadotropin-Releasing Hormone Neuroblastoma Transforming Growth Factor beta Cell Clone Internal medicine Gastrins Blocking antibody Tumor Cells Cultured medicine Humans U87 Epidermal Growth Factor Cell growth Growth factor Antibodies Monoclonal Transforming Growth Factor alpha Cell biology medicine.anatomical_structure Endocrinology Neurology Oncology Cell culture biology.protein Neurology (clinical) Antibody Glioblastoma Cell Division |
| Zdroj: | Journal of Neuro-Oncology. 20:67-80 |
| ISSN: | 1573-7373 0167-594X |
| DOI: | 10.1007/bf01057963 |
| Popis: | The influence of five anti-hormone and/or anti-growth factor neutralizing antibodies on the in vitro proliferation of four human astrocytic tumor cell lines (U87, U138, U373, H4) is quantitatively described by means of a new tool which makes it possible to evaluate cell growth and cell clone architecture concomitantly. This tool relies upon the combined use of the digital cell image analyses of Feulgen-stained nuclei and the Delaunay and Voronoi mathematical triangulation and paving techniques. Of the five anti-hormone and/or anti-growth factors tested here, the anti-luteinizing hormone-releasing hormone (LHRH) antibody induced the most marked perturbation in the U138 and U373 cell lines, whereas this role was played by the anti-epidermal growth factor (EGF) antibody in the U87 and H4 cell lines. The anti-gastrin (G) antibody significantly modified the growth and/or cell clone architecture of the U138, U87 and H4 cell lines, as did the anti-transforming growth factor alpha (TGFalpha) antibody. The anti-transforming growth factor beta (TGFbeta) antibody modified the growth and/or cell clone architecture of the four cell lines under study. If the five antibodies are taken into consideration, the results strongly suggest that four (the anti-G, the anti-EGF, the anti-LHRH and the anti-TGFalpha) act as inhibitory agents on some glioma cell line proliferation, while the fifth one, i.e. the anti-TGFbeta, act as a stimulator of cell proliferation, perhaps by abrogating the inhibitory effects of TGFbeta on proliferation. A comparison of cell growth data with cell clone architecture characteristics provided further evidence of some specific influence exercised by a given hormone and/or growth factor on glioma cell proliferation. Indeed, the anti-LHRH antibody caused the most pronounced perturbations in the U138 and U373 cell clone architecture; this feature was observed in the H4 cell line and, to a lesser extent in the U87 one after the anti-EGF antibody had been used. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full text from SpringerLink