Bilberry extracts induce gene expression through the electrophile response element
| Autor: | Jan Øivind Moskaug, Harald Carlsen, Rune Blomhoff, Kanae Ebihara, Karin Haffner, Line Glemmestad, Mari C. W. Myhrstad, Linn Ingrid Dahl |
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| Rok vydání: | 2006 |
| Předmět: |
Transcriptional Activation
Cancer Research Antioxidant Carcinoma Hepatocellular medicine.medical_treatment Response element Flavonoid Medicine (miscellaneous) Gene Expression Vaccinium myrtillus Biology Response Elements Transfection Transactivation chemistry.chemical_compound Cell Line Tumor Gene expression Chlorocebus aethiops medicine Animals Humans Promoter Regions Genetic Transcription factor chemistry.chemical_classification Nutrition and Dietetics Plant Extracts Liver Neoplasms Promoter Oncology Biochemistry chemistry Fruit COS Cells Quercetin Proto-Oncogene Proteins c-fos |
| Zdroj: | Nutrition and cancer. 54(1) |
| ISSN: | 0163-5581 |
| Popis: | A number of genes important for detoxification and antioxidant defense induced by mild stress generated by, for example, physical activity/exercise, caloric restriction, or alcohol may provide health benefits by causing the organism to mount such a defense response. More recently, induction of these defenses has also been attributed to phytochemicals or secondary metabolites from dietary plants. Many polyphenols, which constitute a large fraction of these phytochemicals, increase cellular levels of antioxidants, such as glutathione and other components of the detoxification systems, via the transactivation of genes containing electrophile response elements (EpREs) within their promoters. One such gene, gamma-glutamylcysteine synthetase, has previously been shown to be positively regulated by quercetin, a flavonoid found in high concentrations in onions, apples, and bilberries through EpRE transactivation. As a further step, we have investigated whether bilberries and quercetin have the ability to induce transcription of Fos-related antigen 1 (Fra-1), which contains two EpREs in its promoter. Fra-1 is a member of the activator protein 1 (AP-1) family of transcription factors and, due to the lack of transactivation domain Fra-1, can suppress activation of AP-1. We present results demonstrating that extracts from bilberries, and the flavonoid quercetin, abundant in bilberries, induce the fra-1 promoter and the cellular content of Fra-1 mRNA. We further provide evidence that this induction is mediated through EpREs. |
| Databáze: | OpenAIRE |
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