Conditioned media of glial cell lines induce alkaline phosphatase activity in cultured artery endothelial cells Identification of interleukin-6 as an induction factor
| Autor: | Mamiko Hosokawa, Misao Ide, Kazue Kaneda, Hisashi Fukushima, Hiroshi Takemoto |
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| Rok vydání: | 1994 |
| Předmět: |
Endothelium
Molecular Sequence Data Biophysics CHO Cells Pulmonary Artery Blood–brain barrier Biochemistry Cell Line Endothelial cell Blood—brain barrier Structural Biology Cricetinae Tumor Cells Cultured Genetics medicine Animals Humans Amino Acid Sequence Enzyme inducer Interleukin 6 Molecular Biology Cells Cultured biology Interleukin-6 Chinese hamster ovary cell Glioma Cell Biology Alkaline Phosphatase Molecular biology Recombinant Proteins Endothelial stem cell medicine.anatomical_structure Cell culture Differentiation Culture Media Conditioned Enzyme Induction biology.protein Alkaline phosphatase Cattle Endothelium Vascular Neuroglia |
| Zdroj: | FEBS Letters. 350:99-103 |
| ISSN: | 0014-5793 |
| Popis: | Conditioned media of human glial cell lines induced alkaline phosphatase activity in cultured calf artery endothelial cells. The maximal alkaline phosphatase activity in the culture was comparable to the level in isolated brain capillary endothelial cells. An induction factor in the conditioned media was purified and identified as interleukin-6 from its amino-terminal sequence, molecular weight, amino acid composition and immunoreactivity. Recombinant interleukin-6 had similar induction activity. Our findings raise the possibility that interleukin-6 induces and modulates alkaline phosphatase activity in endothelial cells during normal development of the blood—brain barrier and under certain pathological conditions. |
| Databáze: | OpenAIRE |
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