Intrinsic Raman spectroscopy for quantitative biological spectroscopy Part I: Theory and simulations

Autor: Kate L. Bechtel, Wei-Chuan Shih, Michael S. Feld
Jazyk: angličtina
Rok vydání: 2008
Předmět:
Popis: We present a novel technique, intrinsic Raman spectroscopy (IRS), to correct turbidity-induced Raman spectral distortions, resulting in the intrinsic Raman spectrum that would be observed in the absence of scattering and absorption. We develop an expression relating the observed and intrinsic Raman spectra through diffuse reflectance using the photon migration depiction of light transport. Numerical simulations are employed to validate the theoretical results and study the dependence of this expression on sample size and elastic scattering anisotropy.
Databáze: OpenAIRE