Immobilization of Cell-Adhesive Laminin Peptides in Degradable PEGDA Hydrogels Influences Endothelial Cell Tubulogenesis
| Autor: | Saniya Ali, Jennifer E. Saik, Dan J. Gould, Mary E. Dickinson, Jennifer L. West |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2013 |
| Předmět: |
Angiogenesis
extracellular matrix lcsh:Medicine 02 engineering and technology General Biochemistry Genetics and Molecular Biology Extracellular matrix 03 medical and health sciences angiogenesis Tissue engineering Laminin Original Research Articles medicine Cell adhesion lcsh:QH301-705.5 030304 developmental biology 0303 health sciences biology Chemistry lcsh:R technology industry and agriculture 021001 nanoscience & nanotechnology Endothelial stem cell medicine.anatomical_structure lcsh:Biology (General) tissue engineering Immunology Self-healing hydrogels Biophysics biology.protein peptides Pericyte 0210 nano-technology biomaterials |
| Zdroj: | BioResearch Open Access, Vol 2, Iss 4, Pp 241-249 (2013) BioResearch Open Access |
| ISSN: | 2164-7860 |
| DOI: | 10.1089/BIORES.2013.0021 |
| Popis: | Attachment, spreading, and organization of endothelial cells into tubule networks are mediated by interactions between cells in the extracellular microenvironment. Laminins are key extracellular matrix components and regulators of cell adhesion, migration, and proliferation. In this study, laminin-derived peptides were conjugated to poly(ethylene glycol) (PEG) monoacrylate and covalently incorporated into degradable PEG diacrylate (PEGDA) hydrogels to investigate the influence of these peptides on endothelial cellular adhesion and function in organizing into tubule networks. Degradable PEGDA hydrogels were synthesized by incorporating a matrix metalloproteinase (MMP)–sensitive peptide, GGGPQGIWGQGK (abbreviated PQ), into the polymer backbone. The secretion of MMP-2 and MMP-9 by endothelial cells promotes polymer degradation and consequently cell migration. We demonstrate the formation of extensive networks of tubule-like structures by encapsulated human umbilical vein endothelial cells in hydrogels with immobilized synthetic peptides. The resulting structures were stabilized by pericyte precursor cells (10T1/2s) in vitro. During tubule formation and stabilization, extracellular matrix proteins such as collagen IV and laminin were deposited. Tubules formed in the matrix of metalloproteinase sensitive hydrogels were visualized from 7 days to 4 weeks in response to different combination of peptides. Moreover, hydrogels functionalized with laminin peptides and transplanted in a mouse cornea supported the ingrowth and attachment of endothelial cells to the hydrogel during angiogenesis. Results of this study illustrate the use of laminin-derived peptides as potential candidates for modification of biomaterials to support angiogenesis. |
| Databáze: | OpenAIRE |
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