Use of a Penetratin-Linked Peptide in Dictyostelium
| Autor: | W. Jonathan Ryves, Adrian J. Harwood |
|---|---|
| Rok vydání: | 2006 |
| Předmět: |
Spores
Protozoan Bioengineering Peptide Cell-Penetrating Peptides Applied Microbiology and Biotechnology Biochemistry Dictyostelium discoideum Animals Dictyostelium Protein kinase A Molecular Biology Cell Aggregation chemistry.chemical_classification Oligopeptide biology Oligonucleotide fungi biology.organism_classification Cyclic AMP-Dependent Protein Kinases Cell aggregation Cell biology chemistry Cytoplasm Mutation Carrier Proteins Peptides Biotechnology |
| Zdroj: | Molecular Biotechnology. 33:123-132 |
| ISSN: | 1073-6085 |
| DOI: | 10.1385/mb:33:2:123 |
| Popis: | The plasma membrane is an effective barrier to most macromolecules and hydrophilic molecules. Remarkably, a class of positively charged cell-penetrating peptides (CPPs) has been discovered that can translocate themselves and associated cargoes into the cytoplasm. These have been used to carry oligopeptide- and oligonucleotide-based inhibitors into mammalian cells. A recent report indicates that the same CPPs are internalized by plant protoplasts, suggesting that this may be a universal phenomenon. We report here that the prototypical CPP, penetratin, enters cells of the free-living amoebae Dictyostelium discoideum. To investigate the functionality of this technology, we fused the penetratin sequence to PKI, a peptide inhibitor of the cAMP-dependent protein kinase (PKA). Consistent with its PKA inhibitory action, Penetratin-PKI blocked aggregation in wild-type cells and, at appropriate concentrations, rescued the phenotype of a Dictyostelium mutant that has constitutively high PKA activity. This technology offers an effective method for delivery of oligopeptides and oligonucleotides into Dictyostelium. |
| Databáze: | OpenAIRE |
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