Influence of nonylphenol from multilayer plastic films on artificial insemination of sows
| Autor: | Qi-Zhi Su, Paula Vera, R. Ausejo, Cristina Nerín, Noelia Mendoza |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
Male
endocrine system Swine medicine.medical_treatment Semen 02 engineering and technology 01 natural sciences Biochemistry Analytical Chemistry law.invention chemistry.chemical_compound Animal science Phenols law medicine Animals Leaching (agriculture) Insemination Artificial Plastic bag urogenital system Chemistry Artificial insemination 010401 analytical chemistry Extender 021001 nanoscience & nanotechnology Sperm 0104 chemical sciences Nonylphenol Semen Analysis Livestock farming Fertility Female 0210 nano-technology Plastics |
| Zdroj: | Analytical and bioanalytical chemistry. 412(24) |
| ISSN: | 1618-2650 |
| Popis: | Artificial insemination is common practice in mass livestock farming. Recently, it was shown that chemicals leaching from multilayer plastic bags affect the fertility of boars, although common quality tests did not show any impact on the sperm. It is not clear whether this incidence was a single case or whether it could be a systematic problem. Therefore, we studied six multilayer plastic bags. A total of 49 compounds were found, but most of them were at very low intensity. Nonylphenols in the range of 19-99 μg/g plastic were found. Migration tests using water and 10% ethanol as simulants, to mimic the behavior of semen with the extender, were performed. The most interesting migrants in terms of potential reprotoxicity were identified as nonylphenols. The identification in depth demonstrated the presence of 10 isomers of nonylphenol with a total concentration range between 16 to 58 μg/Kg simulant, among other migrants at lower concentration. The influence of these nonylphenols and their maximum tolerable concentration in direct contact with semen from boars was studied. Motility, viability, mitochondrial activity and acrosomes reacted were significantly affected at 10 mg/Kg of nonylphenols in contact with the sperm, but in vitro penetration rate was significantly decreased with only 2 mg/Kg. Insight into the mode of action is also provided. |
| Databáze: | OpenAIRE |
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