Improvement of DNA transfer frequency and transposon mutagenesis of Erwinia carotovora subsp. betavasculorum
| Autor: | M Rella, Albert R. Weinhold, P E Axelrood, Milton N. Schroth |
|---|---|
| Rok vydání: | 1989 |
| Předmět: |
Transposable element
DNA Bacterial Ethyl methanesulfonate Mutant Restriction Mapping Mutagenesis (molecular biology technique) Erwinia Applied Microbiology and Biotechnology Microbiology chemistry.chemical_compound Plasmid Genetics Ecology biology Genetic transfer food and beverages Nucleic Acid Hybridization biology.organism_classification Anti-Bacterial Agents chemistry Mutation DNA Transposable Elements bacteria Transposon mutagenesis Food Science Biotechnology Plasmids Research Article |
| Zdroj: | Applied and environmental microbiology. 55(4) |
| ISSN: | 0099-2240 |
| Popis: | The production of antibiotics and their role in microbial competition under natural conditions can be readily studied by the use of transposon mutants. Several antibiotic-producing strains of Erwinia carotovora subsp. betavasculorum were unable to accept foreign DNA. A plasmid delivery system was developed, using ethyl methanesulfonate mutagenesis, which entailed isolating E. carotovora subsp. betavasculorum mutants able to accept foreign DNA and transfer it to other strains. This enabled transposon mutagenesis of a wild-type antibiotic-producing strain of E. carotovora subsp. betavasculorum. Twelve antibiotic-negative mutants were isolated, and one of these showed a reduction in antibiotic production in vitro. Many of these mutants also showed a reduction in their ability to macerate potato tissue. The mutants were classified into four genetic groups on the basis of their genetic and phenotypic characteristics, indicating that several genes are involved in antibiotic biosynthesis by E. carotovora subsp. betavasculorum. |
| Databáze: | OpenAIRE |
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