Normal peroxisome development from vesicles induced by truncated Hansenula polymorpha Pex3p
| Autor: | Klaas Nico Faber, Marten Veenhuis, Richard J.S. Baerends, Anita M. Kram, Gert Jan Haan |
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| Přispěvatelé: | Groningen Biomolecular Sciences and Biotechnology, Molecular Cell Biology, Molecular Genetics, Faculteit Medische Wetenschappen/UMCG, Groningen Institute for Gastro Intestinal Genetics and Immunology (3GI), Center for Liver, Digestive and Metabolic Diseases (CLDM) |
| Jazyk: | angličtina |
| Rok vydání: | 2002 |
| Předmět: |
Saccharomyces cerevisiae Proteins
Nuclear Envelope BIOGENESIS SELECTIVE INACTIVATION ENDOPLASMIC-RETICULUM DEFICIENT MUTANTS Biochemistry Pichia Pichia pastoris Fungal Proteins Peroxins DIHYDROXYACETONE SYNTHASE medicine Peroxisomes Nuclear membrane ALCOHOL OXIDASE Molecular Biology DNA Primers chemistry.chemical_classification biology Base Sequence TARGETING SIGNAL Endoplasmic reticulum Vesicle Membrane Proteins Cell Biology Compartment (chemistry) Peroxisome biology.organism_classification Amino acid medicine.anatomical_structure PICHIA-PASTORIS chemistry Microscopy Fluorescence N-TERMINUS ATP-Binding Cassette Transporters MEMBRANE Biogenesis |
| Zdroj: | The Journal of Biological Chemistry, 277(13), 11026-11033. AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC |
| ISSN: | 0021-9258 |
| Popis: | We show that the synthesis of the N-terminal 50 amino acids of Pex3p (Pex3p(1-50)) in Hansenula polymorpha pex3 cells is associated with the formation of vesicular membrane structures. Biochemical and ultrastructural findings suggest that the nuclear membrane is the donor membrane compartment of these vesicles. These structures also contain Pex14p and can develop into functional peroxisomes after subsequent reintroduction of the full-length Pex3p protein. We discuss the significance of this finding in relation to peroxisome reintroduction, e.g. in case peroxisomes are lost due to failure in inheritance. |
| Databáze: | OpenAIRE |
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