Extraction, structural characterization, and immunoregulatory effect of a polysaccharide fraction from Radix Aconiti Lateralis Preparata (Fuzi)
Autor: | Yihan Wu, Xiaoqin Yang, Fu Shu, Chaomei Fu, Jinming Zhang, Chen Zhang |
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Rok vydání: | 2020 |
Předmět: |
Spleen
Fraction (chemistry) 02 engineering and technology Pharmacology Polysaccharide Plant Roots Biochemistry Radix Aconiti Immunomodulation Mice 03 medical and health sciences Phagocytosis Functional food Polysaccharides Structural Biology Oral administration medicine Animals Immunologic Factors Secretion Rheum Molecular Biology 030304 developmental biology chemistry.chemical_classification 0303 health sciences Aconitum Plant Extracts Macrophages Extraction (chemistry) General Medicine 021001 nanoscience & nanotechnology Rats RAW 264.7 Cells medicine.anatomical_structure chemistry Diterpenes 0210 nano-technology Drugs Chinese Herbal |
Zdroj: | International Journal of Biological Macromolecules. 143:314-324 |
ISSN: | 0141-8130 |
Popis: | A water-soluble polysaccharide fraction named FZPS-1 was obtained from Radix Aconiti Lateralis Preparata (Fuzi in Chinese). Physicochemical and instrumental analyses indicated that the purified FZPS-1 with an average molecular weight of 6.29 × 106 Da was composed of D-arabinose and d -glucose with a molar ratio of 7.5:92.5. It would contain the main chain fragments of →4)-α-D-Glc-(1 → 4)-α-D-Glc-(1→, with the side chain of terminal α-L-Ara-(1 → linked at C6 of D-Glc skeleton. FZPS-1 exhibited significant antioxidative activity and immunomodulatory activity, wherein it promotes macrophage phagocytosis and increases the secretion of macrophage-derived biological factors in RAW 264.7 cells, and a cyclophosphamide-induced immunosuppressed model in a dose-dependent manner. Oral administration of FZPS-1 ameliorated chronic diarrhea in rhubarb-induced spleen deficiency rats and was related to its immunomodulatory activity. Therefore, the obtained FZPS-1 could be potentially utilized as a natural immunomodulatory agent in functional food supplements or drugs. |
Databáze: | OpenAIRE |
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