Isolation and Characterization of Δ6-Desaturase, an ELO-Like Enzyme and Δ5-Desaturase from the Liverwort Marchantia Polymorpha and Production of Arachidonic and Eicosapentaenoic Acids in the Methylotrophic Yeast Pichia Pastoris
| Autor: | Masataka Kajikawa, Eiji Sakuradani, Kanji Ohyama, Hideya Fukuzawa, Katsuyuki T. Yamato, Masutoshi Nojiri, Yoshito Kohzu, Yasuyoshi Sakai, Sakayu Shimizu |
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| Rok vydání: | 2004 |
| Předmět: |
Fatty Acid Desaturases
Hepatophyta Molecular Sequence Data Plant Science Linoleoyl-CoA Desaturase Gene Expression Regulation Enzymologic Mass Spectrometry Pichia Substrate Specificity Pichia pastoris Acyl-CoA chemistry.chemical_compound Marchantia polymorpha Delta-5 Fatty Acid Desaturase Acetyl Coenzyme A Genetics Amino Acid Sequence Phylogeny chemistry.chemical_classification Arachidonic Acid Sequence Homology Amino Acid biology alpha-Linolenic Acid Fatty acid General Medicine biology.organism_classification Recombinant Proteins Eicosapentaenoic Acid chemistry Biochemistry Fatty Acids Unsaturated Fatty acid elongation Linoleoyl-CoA desaturase Agronomy and Crop Science Polyunsaturated fatty acid |
| Zdroj: | Plant Molecular Biology. 54:335-352 |
| ISSN: | 0167-4412 |
| Popis: | The liverwort Marchantia polymorpha contains high proportions of arachidonic and eicosapentaenoic acids. In general, these C20 polyunsaturated fatty acids (PUFA) are synthesized from linoleic and alpha -linolenic acids, respectively, by a series of reactions catalyzed by Delta(6)-desaturase, an ELO-like enzyme involved in Delta(6) elongation and Delta(5)-desaturase. Here we report the isolation and characterization of the cDNAs, MpDES6, MpELO1 and MpDES5, coding for the respective enzymes from M. polymorpha. Co-expression of the MpDES6, MpELO1 and MpDES5 cDNAs resulted in the accumulation of arachidonic and eicosapentaenoic acids in the methylotrophic yeast Pichia pastoris. Interestingly, Delta(6) desaturation by the expression of the MpDES6 cDNA appears to occur both in glycerolipids and the acyl-CoA pool, although other lower-plant Delta(6)-desaturases are known to have a strong preference for glycerolipids. |
| Databáze: | OpenAIRE |
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