Site-specific integration of the Haemophilus influenzae bacteriophage HP1: location of the boundaries of the phage attachment site
| Autor: | Michael A. Hauser, J J Scocca |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 1992 |
| Předmět: |
DNA
Bacterial Operon Molecular Sequence Data medicine.disease_cause Microbiology Haemophilus influenzae Bacteriophage chemistry.chemical_compound Plasmid RNA Transfer medicine Coding region Bacteriophages Molecular Biology Gene Genetics Recombination Genetic biology Base Sequence biology.organism_classification RNA Bacterial chemistry Transfer RNA Attachment Sites Microbiological DNA Viral DNA Research Article Plasmids |
| Popis: | Plasmids containing DNA segments from the attachment region of phage HP1 were constructed and tested for the ability to replace the phage attachment site substrate in site-specific recombination reactions. The distance separating the boundaries of the functional site was 418 bp. Replacements within the 11-residue segment 5'-GGCGGTTATCG at the left boundary or within the 12-residue segment 5'-GGATTTTTTGAA at the right boundary abolished substrate activity. A segment of the 418-residue sequence preserves the integrity of an operon of three Haemophilus influenzae tRNA genes after HP1 insertion within the coding sequence. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|