Metabolic pathways of isolated Malpighian tubules of the blowfly functioning in an artificial medium
| Autor: | M.J. Berridge |
|---|---|
| Rok vydání: | 1966 |
| Předmět: |
Malpighian tubule system
Insecta food.ingredient Antimetabolites Physiology Pentoses Oligosaccharides Oxidative phosphorylation Malpighian Tubules Urine Biology Calliphora food Culture Techniques Yolk Methods Animals Hexoses chemistry.chemical_classification fungi biology.organism_classification Culture Media Amino acid Cell biology Metabolic pathway Tubule chemistry Insect Science Dinitrophenol |
| Zdroj: | Journal of Insect Physiology. 12:1523-1538 |
| ISSN: | 0022-1910 |
| Popis: | Malpighian tubules of Calliphora will survive in an artificial medium for 60 hr. The rate of urine production varies considerably during adult life and can be correlated with events during the oviposition cycle. Tubules showing highest rates of urine flow were obtained from insects immediately after emergence when large volumes of fluid are being excreted. Tubules removed from insects during yolk deposition had the lowest rates. Tubules lack an endogenous supply of energy but are capable of using a wide variety of substrates, including various oligosaccharides, hexoses, phosphorylated compounds, organic, and amino acids. Consideration of these indicates that tubule cells probably possess a classical glycolytic-Krebs cycle pathway. Sensitivity of tubule cells to cyanide, azide, and dinitrophenol also indicates that energy production is geared to oxidative phosphorylation. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|