Comparative study of sodium bicarbonate- and magnesium hydroxide-based gastric antacids for the effectiveness of Salmonella delivered Brucella antigens against wild type challenge in BALB/c mice

Autor: John Hwa Lee, Amal Senevirathne, Chamith Hewawaduge, Myeon-Sik Yang, Bumseok Kim, Tae-Won Jeong
Rok vydání: 2021
Předmět:
Salmonella typhimurium
Microbiology (medical)
Salmonella
Magnesium Hydroxide
Drug Compounding
medicine.medical_treatment
Brucella Vaccine
Brucella abortus
chemistry.chemical_element
Buffers
Pharmacology
Vaccines
Attenuated
medicine.disease_cause
Brucellosis
BALB/c
Gastric Acid
Mice
03 medical and health sciences
chemistry.chemical_compound
Immune system
Oral administration
Antacid
medicine
Animals
Immunology and Allergy
030304 developmental biology
Antigens
Bacterial
Mice
Inbred BALB C
0303 health sciences
Attenuated vaccine
Sodium bicarbonate
General Immunology and Microbiology
biology
030306 microbiology
Chemistry
Magnesium
Immunity
General Medicine
Hydrogen-Ion Concentration
biology.organism_classification
Specific Pathogen-Free Organisms
Sodium Bicarbonate
Infectious Diseases
Bacterial Vaccines
Models
Animal
Female
Antacids
Zdroj: Pathogens and Disease. 79
ISSN: 2049-632X
Popis: We compared the effects of two antacid formulations based on sodium bicarbonate and magnesium hydroxide on a Salmonella-delivered oral Brucella live attenuated vaccine. We conducted a series of in vitro and in vivo experiments to investigate the pH buffering capacity, buffering longevity and the effects of these formulations on the survival of Salmonella under neutralized pH conditions and its impact on immune responses. Magnesium hydroxide had a greater, stable and prolonged buffering capacity than sodium bicarbonate and was safer when administered orally. Oral administration of sodium bicarbonate resulted in discomfort as reflected by mouse behavior and mild muscle tremors, whereas mice treated with magnesium hydroxide and PBS were completely normal. Gastric survival studies using BALB/c mice revealed that a higher number of Salmonella reached the intestine when the magnesium hydroxide-based antacid buffer was administrated. Co-administration with attenuated Salmonella secreting Brucella antigens, SodC and Omp19 along with individual antacid formulations, significantly enhanced the antigen-specific protective immune responses against virulent Brucella challenge. Together, our results indicated that the pre vaccinated oral administration of bicarbonate-citric acid or magnesium hydroxide-based neutralizing buffers significantly counteract stomach acidity by maintaining the viability of an oral enteric vaccine formulation.
Databáze: OpenAIRE
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