| Popis: |
Various techniques, such as peptide chemistry, DNA origami, or nanotubes cutting, have been tried to develop synthetic analogues of biological membrane channels for transmembrane transport of ions and molecules, but challenges still remain to achieve the desired affinity, transport properties and biocompatibility for delivery applications. Here we report the development of CNT porins, an ultra-short biocompatible carbon nanotube with the dimension of 5-15 nm in length and 1.5 nm in diameters and with structure and function resembling membrane channel. We explored concentration-dependent interaction of the CNT porins with CHO cells using microscopy and OmniLog phenotype microarray system. Interestingly, the CNT porins didn’t inhibit the cell viability at low concentration over a 72 hr co-incubation period. Single channel recording experiments also showed well-defined channels formation on the cell membrane. Those results indicated those artificial transmembrane channels could be established as a promising biomimetic platform for developing cell interfaces, studying transport in biological channels, and creating stochastic sensors. Their inherent robustness towards biological and chemical challenges and exceptional biocompatibility should prove valuable for bionanofluidic and cellular interface applications. |
