The Ratio of Plasma and Urinary 8-oxo-Gsn Could Be a Novel Evaluation Index for Patients with Chronic Kidney Disease
| Autor: | Li Qun Zhang, Ban Zhao, Yong Hui Mao, Sadayoshi Ito, Leng Nan Xu, Zhe Chen, Qing Hua Weng, Ming Zhang Zuo, Jian Ping Cai, Xiang-yu Li, Ji Hong Hu, Jing Jing Nie |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
Adult
Male 0301 basic medicine Aging medicine.medical_specialty Article Subject Urinary system Urology Renal function Urine medicine.disease_cause Biochemistry Young Adult 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Tandem Mass Spectrometry medicine Humans Deoxyguanosine heterocyclic compounds Renal Insufficiency Chronic lcsh:QH573-671 Aged Aged 80 and over Creatinine Guanosine lcsh:Cytology business.industry Cell Biology General Medicine Middle Aged medicine.disease Pathophysiology 030104 developmental biology chemistry Female Reactive Oxygen Species business 030217 neurology & neurosurgery Oxidative stress Research Article Chromatography Liquid Kidney disease |
| Zdroj: | Oxidative Medicine and Cellular Longevity, Vol 2018 (2018) Oxidative Medicine and Cellular Longevity |
| ISSN: | 1942-0994 1942-0900 |
| DOI: | 10.1155/2018/4237812 |
| Popis: | Nucleic acid oxidation plays an important role in the pathophysiology progress of a variety of diseases. 8-oxo-7,8-dihydro-2′-deoxyguanosine (8-oxo-dGsn) and 8-oxo-7,8-dihydroguanosine (8-oxo-Gsn), which originate from DNA and RNA oxidation, were the most widely used indicators for oxidative stress. The study investigated the relation between 8-oxo-dGsn, 8-oxo-Gsn, and CKD. 146 patients with CKD were divided into five disease stages, and their fasting blood and morning urine were collected. The levels of 8-oxo-dGsn and 8-oxo-Gsn in plasma and urine were quantified by LC-MS/MS. The ratio of urinary 8-oxo-Gsn to creatinine increased from stages 1 to 4 corresponding to the increased severity of CKD, but it decreased in stage 5. And plasma 8-oxo-Gsn gradually increased with the decline of renal function. In particular, the increased ratio of plasma and urine 8-oxo-Gsn in stage 5 exceeded the concentration of creatinine. This trend was similar to the estimated glomerular filtration rate (eGFR), which indicates that 8-oxo-Gsn could be an appropriate indicator for renal function. Our finding indicates that as the disease progresses, RNA oxidation is increased. The significant increase in the ratio of plasma and urinary 8-oxo-Gsn is a novel evaluation index of end-stage renal disease. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Plný text