Actin microfilaments are essential for the cytological positioning and morphology of the Golgi complex
| Autor: | Ferran Valderrama, Teresa Babia, Jan Willem Kok, Inmaculada Ayala, Jaime Renau-Piqueras, Gustavo Egea |
|---|---|
| Přispěvatelé: | Center for Liver, Digestive and Metabolic Diseases (CLDM) |
| Rok vydání: | 1998 |
| Předmět: |
Histology
ENDOPLASMIC-RETICULUM Golgi Apparatus MOLECULAR MOTORS Biology Microfilament Kidney Microtubules Pathology and Forensic Medicine chemistry.chemical_compound symbols.namesake Microtubule MAMMALIAN-CELLS POLARIZED EPITHELIAL-CELLS Animals Secretory pathway Cells Cultured Cytoskeleton RAT HEPATOCYTES Endoplasmic reticulum INTERNALIZATION STEP Cell Biology General Medicine Brefeldin A Golgi apparatus BASOLATERAL ENDOCYTOSIS Actin cytoskeleton Actins Cell biology Golgi complex secretory pathway Rats Actin Cytoskeleton chemistry Golgi cisterna symbols CACO-2 CELLS actin CYTOCHALASIN-D RECEPTOR-MEDIATED ENDOCYTOSIS |
| Zdroj: | European Journal of Cell Biology, 76(1), 9-17. ELSEVIER GMBH, URBAN & FISCHER VERLAG Europe PubMed Central Scopus-Elsevier |
| ISSN: | 0171-9335 |
| Popis: | The organization and function of the Golgi complex was studied in normal rat kidney cells following disruption of the actin cytoskeleton induced by cytochalasin D. In cells treated with these reagents, the reticular and perinuclear Golgi morphology acquired a cluster shape restricted to the centrosome region. Golgi complex alteration affected all Golgi subcompartments as revealed by double fluorescence staining with antibodies to the cis/middle Mannosidase II and the trans-Golgi network TGN38 proteins or vital staining with the lipid derivate C-6-NBD-ceramide. The ultrastructural and stereological analysis showed that the Golgi cisternae remained attached in a stacked conformation, but they were swollen and contained electron-dense intra-cisternal bodies, The Golgi complex cluster remained linked to microtubules since it was fragmented and dispersed after treatment with nocodazole. Moreover, the reassembly of Golgi fragments after the disruption of the microtubuli with nocodazole does not utilize the actin microfilaments. The actin microfilament requirement for the disassembly and reassembly of the Golgi complex and for the ER-Golgi vesicular transport were also studied. The results show that actin microfilaments are not needed for either the retrograde fusion of the Golgi complex with the endoplasmic reticulum promoted by brefeldin A or the anterograde reassembly after the removal of the drug, or the ER-Golgi transport of VSV-G glycoprotein. However, actin microfilaments are directly involved in the subcellular localization and the morphology of the Golgi complex. |
| Databáze: | OpenAIRE |
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