Going further post-RNA-seq: In silico functional analyses revealing candidate genes and regulatory elements related to mastitis in dairy cattle
| Autor: | L.L. Verardo, Ana Paula Sbardella, Hyago Passe Pereira, Mayara Morena Del Cambre Amaral Weller, Marco Antonio Machado, Danísio Prado Munari, Raquel Morais de Paiva Daibert, W. A. Carvalho, Marta Fonseca Martins |
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| Přispěvatelé: | Universidade Federal de Juiz de Fora, Universidade Federal Dos Vales Do Jequitinhonha e Mucuri, Universidade Federal do Espírito Santo (UFES), Universidade Estadual Paulista (UNESP), Empresa Brasileira de Pesquisa Agropecuária (EMBRAPA) |
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: | |
| Zdroj: | Scopus Repositório Institucional da UNESP Universidade Estadual Paulista (UNESP) instacron:UNESP |
| Popis: | Made available in DSpace on 2022-04-28T19:42:49Z (GMT). No. of bitstreams: 0 Previous issue date: 2021-08-01 This study aimed to obtain a better understanding of the regulatory genes and molecules involved in the development of mastitis. For this purpose, the transcription factors (TF) and MicroRNAs (miRNA) related to differentially expressed genes previously found in extracorporeal udders infected with Streptococcus agalactiae were investigated. The Gene-TF network highlighted LOC515333, SAA3, CD14, NFKBIA, APOC2 and LOC100335608 and genes that encode the most representative transcription factors STAT3, PPARG, EGR1 and NFKB1 for infected udders. In addition, it was possible to highlight, through the analysis of the gene-miRNA network, genes that could be post-transcriptionally regulated by miRNAs, such as the relationship between the CCL5 gene and the miRNA bta-miR-363. Overall, our data demonstrated genes and regulatory elements (TF and miRNA) that can play an important role in mastitis resistance. The results provide new insights into the first functional pathways and the network of genes that orchestrate the innate immune responses to infection by Streptococcus agalactiae. Our results will increase the general knowledge about the gene networks, transcription factors and miRNAs involved in fighting intramammary infection and maintaining tissue during infection and thus enable a better understanding of the pathophysiology of mastitis. Institute of Biological Sciences Universidade Federal de Juiz de Fora Zootechnics Department Universidade Federal Dos Vales Do Jequitinhonha e Mucuri Zootechnics Department Universidade Federal Do Espírito Santo Department of Exact Sciences Universidade Estadual Paulista Júlio de Mesquita Filho Molecular Genetics Laboratory Embrapa Gado de Leite Department of Exact Sciences Universidade Estadual Paulista Júlio de Mesquita Filho |
| Databáze: | OpenAIRE |
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