Multiple field and glasshouse assessments increase the reliability of linkage mapping of the Vf source of applescab resistance in apple

Autor: Graham J.W. King, Chris Maliepaard, A.G. Manganaris, J.J. Verhaegh, Kate Evans, R. Vrielink, P. Roche, François Laurens, F. H. Alston, James R. Lynn, Frank Dunemann, Stefano Tartarini, H. Schmidt, E. Chevreau, L.M. Brown, J. Janse
Přispěvatelé: Unité mixte de recherche génétique et horticulture Genhort, Université d'Angers (UA)-Institut National de la Recherche Agronomique (INRA)-Institut National d'Horticulture, ProdInra, Migration
Jazyk: angličtina
Rok vydání: 1998
Předmět:
0106 biological sciences
Population
Centrum voor Plantenveredelings- en Reproduktieonderzoek
Context (language use)
[SDV.GEN] Life Sciences [q-bio]/Genetics
01 natural sciences
Wiskundige en Statistische Methoden - Biometris
03 medical and health sciences
Cluster analysis
Scab resistance
Gene mapping
Genetics
education
Mathematical and Statistical Methods - Biometris
ComputingMilieux_MISCELLANEOUS
030304 developmental biology
2. Zero hunger
0303 health sciences
education.field_of_study
[SDV.GEN]Life Sciences [q-bio]/Genetics
biology
Venturia inaequalis
Apple
Venturia
General Medicine
biology.organism_classification
RAPD
Apple scab
Genetic marker
Linkage mapping
Malus
Restriction fragment length polymorphism
Agronomy and Crop Science
RESISTANCE GENETIQUE
010606 plant biology & botany
Biotechnology
Zdroj: TAG Theoretical and Applied Genetics
TAG Theoretical and Applied Genetics, Springer Verlag, 1998, 96, pp.699-708
Theoretical and Applied Genetics 96 (1998)
Theoretical and Applied Genetics, 96, 699-708
ISSN: 0040-5752
1432-2242
DOI: 10.1007/s001220050791
Popis: Apple scab, caused by the fungus Venturia inaequalis (Cke.) Wint., is an important disease in commercial apple production. A mapping population of 155 individuals, derived from a cross between the apple varieties ‘Prima’ (resistant)בFiesta’ (susceptible), was scored for response to the disease in replicated field and glasshouse trials throughout Europe. Twenty data sets were selected and cluster analysis was used to form a consensus score for the population fitting a 1 : 1 segregation ratio of resistance:susceptibility. The progeny were scored with molecular markers. A detailed map covering 54 cM of the ‘Prima’ linkage group containing the Vf gene for scab resistance was constructed using 24 molecular markers linked to the resistance gene. One isoenzyme marker (Pgm-1), six RFLP markers and 17 RAPD markers formed a linkage group with the consensus measure of resistance to scab. Four marker bridges were established with the corresponding ‘Fiesta’ linkage group with additional markers (one isozyme, one RFLP, three RAPD and one AFLP). A low chi-square value indicated a good fit of the marker ordering, which was in close agreement with previously reported linkage positions for some of the markers and Vf. Differences were observed in the ability of different scoring methods to resolve susceptible and resistant classes. The results obtained for the consensus classification of resistance to scab for the population may suggest the presence of virulent inocula at some sites, which could overcome the Vf gene for resistance. The consequences of relying on individual scoring occasions for studying Vf scab resistance are discussed in the context of linkage analysis, conventional breeding selection, and marker-assisted selection.
Databáze: OpenAIRE
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