16S rRNA PCR on clinical specimens: Impact on diagnosis and therapeutic management
| Autor: | Angélique Chapuis, C. de Curraize, N. Dullier-Taillefumier, C. Neuwirth, B. Nicolas, Alain Putot, Julien Bador, Lucie Amoureux, Véronique Varin |
|---|---|
| Přispěvatelé: | Laboratoire de Bactériologie (CHU Dijon), Centre Hospitalier Universitaire de Dijon - Hôpital François Mitterrand (CHU Dijon), Laboratoire de bactériologie (CHU Dijon), Plateau technique de Biologie [CHU de Dijon], Centre Hospitalier Universitaire de Dijon - Hôpital François Mitterrand (CHU Dijon)-Centre Hospitalier Universitaire de Dijon - Hôpital François Mitterrand (CHU Dijon) |
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0303 health sciences
medicine.medical_specialty 030306 microbiology business.industry medicine.drug_class [SDV]Life Sciences [q-bio] Antibiotics Clinical settings Bacterial Infections University hospital 16S ribosomal RNA Laboratory results Polymerase Chain Reaction 3. Good health Discontinuation 03 medical and health sciences Infectious Diseases RNA Ribosomal 16S Internal medicine Antibiotic therapy Humans Medicine Prospective Studies business Retrospective Studies |
| Zdroj: | Médecine et Maladies Infectieuses Médecine et Maladies Infectieuses, Elsevier Masson, 2020, 50, pp.63-73. ⟨10.1016/j.medmal.2019.09.014⟩ |
| ISSN: | 0399-077X |
| DOI: | 10.1016/j.medmal.2019.09.014⟩ |
| Popis: | Objective 16S rRNA PCR (16S PCR) performed on clinical samples contributes to bacterial identification in cases of negative culture due to an antibiotic therapy. Sensitivity of the 16S PCR is low (19–42%). Little data is available on its impact on the management of patients. We aimed to evaluate the contribution of 16S PCR to diagnosis and therapeutic management at the university hospital of Dijon, France. Patients and methods 16S PCR was performed on the clinical specimens of 132 patients. Clinical settings, laboratory results, and data on antibiotic therapy were collected, as well as conclusions drawn from the 16S PCR result by physicians. Each case was analyzed to determine if the 16S PCR was helpful. The relevance of the 16S PCR was also assessed. Results The 16S PCR yield was 27.3%, ranging from 14.3% to 64.3% depending on the type of specimen. 16S PCR had a positive impact on diagnosis in 28.8% of cases. Five negative 16S PCR results were considered helpful as they contributed to ruling out bacterial infection. 16S PCR led to treatment changes in six patients (4.5%): three narrower spectrums, two treatment adaptations, and one discontinuation. The 16S PCR was considered “non-relevant” in 35 cases (26.5%). None of these 35 PCRs contributed to the patient's management. Conclusion Physicians should be aware of performances of 16S PCR. Dialogue between physicians and bacteriologists is essential for appropriate selection of indications and correct interpretation of results. |
| Databáze: | OpenAIRE |
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