Short-term environmental enrichment exposure induces proliferation and maturation of doublecortin-positive cells in the prefrontal cortex
| Autor: | Zhi Li, Lei Shang, Kun Xiong, Ngobe Akume Cynthia, Jufang Huang, Zhenyu Yang, Mengqi Zhang, Dan Chen, Chun-Ling Fan |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2014 |
| Předmět: |
Pathology
medicine.medical_specialty Technical Updates doublecortin protein neurons Biology Guinea pig Andrology Developmental Neuroscience medicine neocortex Prefrontal cortex nerve regeneration NSFC grant Environmental enrichment prefrontal cortex Neocortex 5-bromodeoxyuridine Neurogenesis Doublecortin neurogenesis medicine.anatomical_structure cell proliferation nervous system Cerebral cortex biology.protein NeuN neural regeneration guinea pig |
| Zdroj: | Neural Regeneration Research |
| ISSN: | 1876-7958 1673-5374 |
| Popis: | Previous studies have demonstrated that doublecortin-positive immature neurons exist predominantly in the superficial layer of the cerebral cortex of adult mammals such as guinea pigs, and these neurons exhibit very weak properties of self-proliferation during adulthood under physiological conditions. To verify whether environmental enrichment has an impact on the proliferation and maturation of these immature neurons in the prefrontal cortex of adult guinea pigs, healthy adult guinea pigs were subjected to short-term environmental enrichment. Animals were allowed to play with various cognitive and physical stimulating objects over a period of 2 weeks, twice per day, for 60 minutes each. Immunofluorescence staining results indicated that the number of doublecortin-positive cells in layer II of the prefrontal cortex was significantly increased after short-term environmental enrichment exposure. In addition, these doublecortin-positive cells co-expressed 5-bromo-2-deoxyuridine (a marker of cell proliferation), c-Fos (a marker of cell viability) and NeuN (a marker of mature neurons). Experimental findings showed that short-term environmental enrichment can induce proliferation, activation and maturation of doublecortin-positive cells in layer II of the prefrontal cortex of adult guinea pigs. |
| Databáze: | OpenAIRE |
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