Genes related to mitochondrial functions are differentially expressed in phosphine-resistant and -susceptible Tribolium castaneum
| Autor: | Marcé D. Lorenzen, Yongming Sun, Frank H. Arthur, Michael J. Aikins, Kelli Bramlett, Kelly Hoon, Brenda Oppert, Gavin Meredith, Brandon Blakey, Dalia Dhingra, Thomas W. Phillips, Kun Yan Zhu, Natalie Supunpong Hernandez, Zhaorigetu Chen, Lindsey C. Perkin, Raul Narciso C. Guedes, Madison Taylor, Folukemi Adedipe, Brian A Sanderson, George P. Opit |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2015 |
| Předmět: |
Insecticide resistance
Sequence analysis Phosphines Phosphine resistance Population Molecular Sequence Data Cytochrome P450 Biology Stored product pests Transcriptome Red flour beetle Tribolium castaneum Cytochrome P-450 Enzyme System Gene expression Nitriles Pyrethrins Genetics Animals RNA-Seq Amino Acid Sequence education Anti-diuretic peptide Dihydrolipoamide dehydrogenase Gene Fumigants education.field_of_study Gene knockdown Tribolium Sequence Analysis RNA Genomics biology.organism_classification Mitochondria biology.protein Deltamethrin Biotechnology Research Article |
| Zdroj: | BMC Genomics LOCUS Repositório Institucional da UFV Universidade Federal de Viçosa (UFV) instacron:UFV |
| ISSN: | 1471-2164 |
| Popis: | Background Phosphine is a valuable fumigant to control pest populations in stored grains and grain products. However, recent studies indicate a substantial increase in phosphine resistance in stored product pests worldwide. Results To understand the molecular bases of phosphine resistance in insects, we used RNA-Seq to compare gene expression in phosphine-resistant and susceptible laboratory populations of the red flour beetle, Tribolium castaneum. Each population was evaluated as either phosphine-exposed or no phosphine (untreated controls) in triplicate biological replicates (12 samples total). Pairwise analysis indicated there were eight genes differentially expressed between susceptible and resistant insects not exposed to phosphine (i.e., basal expression) or those exposed to phopshine (>8-fold expression and 90 % C.I.). However, 214 genes were differentially expressed among all four treatment groups at a statistically significant level (ANOVA, p |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|