Regulating T helper cell immunity through antigen responsiveness and calcium entry
| Autor: | Michael G. McHeyzer-Williams, Louise J. McHeyzer-Williams, Rebecca R. Pogue-Caley, Gabriel Bikah |
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| Rok vydání: | 2001 |
| Předmět: |
Antigens
Differentiation T-Lymphocyte CD3 Complex T cell Immunology Receptors Antigen T-Cell Cytochrome c Group In Vitro Techniques Lymphocyte Activation Calcium in biology Mice Antigen CD28 Antigens Calmodulin Antigens CD GTP-Binding Proteins medicine Immunology and Allergy Cytotoxic T cell Animals Lectins C-Type Calcium Signaling Antigens Columbidae Calcium signaling CD40 biology Calcineurin T helper cell T-Lymphocytes Helper-Inducer Cell biology medicine.anatomical_structure Cross-Linking Reagents CD4 Antigens biology.protein Cell Division |
| Zdroj: | Nature immunology. 1(5) |
| ISSN: | 1529-2908 |
| Popis: | We evaluated changes in the signaling potentials and proliferative capacity of single antigen-specific T helper (TH) cells during a primary immune response to a protein antigen. At the peak of cellular expansion in vivo all antigen-specific TH cells exhibited a profound block in CD3- and CD4-mediated mobilization of intracellular calcium together with a more global block in T cell receptor-independent capacitative calcium entry (CCE). The proliferative response of these antigen-specific TH cells to anti-CD3, anti-CD28 and IL-2 was also severely blunted. Cross-linking CD69 on a substantial fraction of CD69+ antigen-specific TH cells relieved this block in CCE and restored proliferative capacity in vitro. The CCE rescue operated through a CD69-coupled G protein and required calcium-bound calmodulin and calcineurin. These data reveal critical changes in the responsiveness of antigen-specific TH cells and provide evidence of new mechanisms for the regulation of antigen-specific TH cell development in vivo. |
| Databáze: | OpenAIRE |
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