In Vivo Interaction Proteomics in Caenorhabditis elegans Embryos Provides New Insights into P Granule Dynamics*
| Autor: | Patricia G. Cipriani, Matthias Selbach, Kristin C. Gunsalus, D. Mecenas, Jolanta Polanowska, Fabio Piano, Jia-Xuan Chen |
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| Přispěvatelé: | Centre d'Immunologie de Marseille - Luminy (CIML), Aix Marseille Université (AMU)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Aix Marseille Université (AMU) |
| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Proteomics Quantitative proteomics Regulator Protein Serine-Threonine Kinases Cytoplasmic Granules Biochemistry Germline Chromatography Affinity Mass Spectrometry Analytical Chemistry Protein–protein interaction 03 medical and health sciences In vivo Animals Protein Interaction Maps Phosphorylation Caenorhabditis elegans Caenorhabditis elegans Proteins Molecular Biology biology Research Gene Expression Regulation Developmental Protein-Tyrosine Kinases biology.organism_classification Cell biology 030104 developmental biology [SDV.IMM]Life Sciences [q-bio]/Immunology Carrier Proteins |
| Zdroj: | Molecular and Cellular Proteomics Molecular and Cellular Proteomics, 2016, 15 (5), pp.1642-1657. ⟨10.1074/mcp.M115.053975⟩ Molecular and Cellular Proteomics, American Society for Biochemistry and Molecular Biology, 2016, 15 (5), pp.1642-1657. ⟨10.1074/mcp.M115.053975⟩ |
| ISSN: | 1535-9476 1535-9484 |
| Popis: | International audience; Studying protein interactions in whole organisms is fundamental to understanding development. Here, we combine in vivo expressed GFP-tagged proteins with quantitative proteomics to identify protein-protein interactions of selected key proteins involved in early C. elegans embryogenesis. Co-affinity purification of interaction partners for eight bait proteins resulted in a pilot in vivo interaction map of proteins with a focus on early development. Our network reflects known biology and is highly enriched in functionally relevant interactions. To demonstrate the utility of the map, we looked for new regulators of P granule dynamics and found that GEI-12, a novel binding partner of the DYRK family kinase MBK-2, is a key regulator of P granule formation and germline maintenance. Our data corroborate a recently proposed model in which the phosphorylation state of GEI-12 controls P granule dynamics. In addition, we find that GEI-12 also induces granule formation in mammalian cells, suggesting a common regulatory mechanism in worms and humans. Our results show that in vivo interaction proteomics provides unique insights into animal development. |
| Databáze: | OpenAIRE |
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