Detection of oyster herpesvirus DNA and proteins in asymptomatic Crassostrea gigas adults
| Autor: | Andre Gerard, Tristan Renault, Anne Thebault, Isabelle Arzul |
|---|---|
| Rok vydání: | 2002 |
| Předmět: |
Cancer Research
Oyster animal structures viruses In situ hybridization Ostrea angasi Adult oyster Genome Virus 03 medical and health sciences Healthy carrier Viral Proteins Virology biology.animal Animals 14. Life underwater Herpesviridae 030304 developmental biology 0303 health sciences biology Protein fungi food and beverages Herpesvirus 04 agricultural and veterinary sciences DNA Herpesviridae Infections Pacific oyster biology.organism_classification equipment and supplies Ostreidae Infectious Diseases Crassostrea gigas DNA Viral 040102 fisheries 0401 agriculture forestry and fisheries Crassostrea Asymptomatic carrier |
| Zdroj: | Virus Research (0168-1702) (Elsevier), 2002-03, Vol. 84, N. 1-2, P. 151-160 |
| ISSN: | 0168-1702 |
| Popis: | Since 1972, several herpes-like virus infections have been reported among different bivalve species around the world. Most of these reports involved larvae or juveniles presenting high mortalities. Two case reports of herpes-like viruses concerned adult oysters, Crassostrea virginica in USA and Ostrea angasi in Australia. Molecular techniques including PCR and in situ hybridization (ISH) have been recently developed to detect the oyster herpesvirus genome. In the present study, 30 Pacific oyster, Crassostrea gigas, adults have been analyzed using three different techniques: PCR, ISH and immunochemistry, in order to detect herpesviruses in asymptomatic individuals. PCR and ISH allowed detection of oyster herpesvirus DNA in 93.3 and 86.6%, respectively, of analyzed oysters while polyclonal antibodies allowed detection of viral proteins in 76.6% of analyzed adult oysters. These results suggest that oyster herpesvirus infects adult oysters with high prevalence and that the virus may persist in its host after primary infection. The detection of viral DNA and viral proteins in the gonad of several individuals supports the hypothesis of a possible vertical transmission of the infection. Lastly, concordance among the three techniques used in this study is discussed. |
| Databáze: | OpenAIRE |
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