Isolation of Bdellovibrio and like organisms and potential to reduce acute hepatopancreatic necrosis disease caused by Vibrio parahaemolyticus
| Autor: | Jetnapang Kongrueng, William P. Robins, John J. Mekalanos, Khotchawan Bangpanwimon, Pimonsri Mitraparp-Arthorn, Varaporn Vuddhakul |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
030106 microbiology Aquatic Science medicine.disease_cause Bdellovibrio Microbiology 03 medical and health sciences Plasmid Penaeidae RNA Ribosomal 16S medicine Animals Phylogeny Ecology Evolution Behavior and Systematics biology Toxin Vibrio parahaemolyticus biology.organism_classification 16S ribosomal RNA Shrimp RNA Bacterial Biofilms Host-Pathogen Interactions Bacteria Photorhabdus |
| Zdroj: | Diseases of Aquatic Organisms. 124:223-232 |
| ISSN: | 1616-1580 0177-5103 |
| DOI: | 10.3354/dao03120 |
| Popis: | Acute hepatopancreatic necrosis disease, a severe disease of shrimp, is caused by Vibrio parahaemolyticus (AHPND Vp), a halophilic bacterium harboring a plasmid that contains toxin genes homologous to Photorhabdus insect-related toxins. We obtained 9 isolates of Bdellovibrio and like organisms (BALOs) from water and sediment samples in Thailand. Using 16S rRNA sequencing, all of the organisms were identified as Bacteriovorax spp. and were able to attack all tested AHPND Vp isolates. In addition, their various susceptible hosts, including Gram-positive and Gram-negative bacteria, were observed. The optimal ratio for interaction between the Bacteriovorax isolate BV-A and AHPND Vp was determined to be 1:10. The suitable conditions applied for co-culture between BV-A and AHPND Vp were 30°C, 2% NaCl, and pH 7.6. The capability of BV-A to reduce numbers of AHPND Vp in vitro was observed in co-culture after incubation for 2 d and continued until the end of the incubation period. In vivo, BV-A was able to reduce mortality of shrimp post-larvae infected with AHPND Vp. In addition, BV-A significantly decreased the formation of biofilm by AHPND Vp. These findings provide evidence for using Bacteriovorax as a biocontrol of AHPND Vp in shrimp aquaculture. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|