Phospholipid-Binding Domain of Factor VIII Is Involved in Endothelial Cell–Mediated Activation of Factor X by Factor IXa
| Autor: | H.-J. M. Brinkman, Jan A. van Mourik, Koen Mertens |
|---|---|
| Rok vydání: | 2002 |
| Předmět: |
Phosphatidylserines
Factor IXa chemistry.chemical_compound Tissue factor medicine Humans Annexin A5 Binding site Cells Cultured Phospholipids Factor IX C2 domain Factor VIII Factor X Antibodies Monoclonal Protein Structure Tertiary Cell biology Endothelial stem cell chemistry Biochemistry Phospholipid Binding Endothelium Vascular Peptides Cardiology and Cardiovascular Medicine medicine.drug |
| Zdroj: | Arteriosclerosis, Thrombosis, and Vascular Biology. 22:511-516 |
| ISSN: | 1524-4636 1079-5642 |
| Popis: | Apparently quiescent, nonapoptotic endothelial cells mediate the activation of factor X by activated factor IX in the presence of its cofactor, activated factor VIII. In a previous study, we reported that during the activation of factor X, the interaction of the cofactor with the endothelial cell membrane clearly differs from the interaction of the cofactor with artificial lipid membranes. In the present study, we identified the peptide domain of factor VIII involved in the assembly of the enzyme-cofactor complex on the endothelial cell surface. With the use of monoclonal antibodies against different peptide sequences on the factor VIII light chain, it was observed that the lipid-binding region of the C2 domain on the factor VIII light chain mediates the assembly of the factor X–activating complex on the endothelial cell surface. In addition, a synthetic peptide that constitutes region Ala2318-Tyr2332 of the C2 domain and that is known for its ability to inhibit the binding of factor VIII to artificial lipid membranes also showed inhibition of the cofactor activity of factor VIII on endothelial cells. Thus, the carboxy-terminal part of the factor VIII light chain not only contains sites involved in lipid binding but also contains sites involved in complex assembly on the endothelial cell membrane. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|