Long non-coding RNA HOTAIR acts as a competing endogenous RNA to promote glioma progression by sponging miR-126-5p
| Autor: | Teng Wan, Wei Tian, Liang Liu, Sitong Cui, Xiaojian Li, Liangsheng Luo, Yan Shi, Rui Zhang |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Male Physiology Carcinogenesis Clinical Biochemistry Mice Nude Biology medicine.disease_cause 03 medical and health sciences Mice 0302 clinical medicine Glutaminase Glioma Cell Line Tumor medicine Animals Humans Hox gene Competing endogenous RNA RNA HOTAIR Cell Biology medicine.disease Long non-coding RNA Up-Regulation Gene Expression Regulation Neoplastic MicroRNAs 030104 developmental biology 030220 oncology & carcinogenesis Cancer research Disease Progression RNA Long Noncoding |
| Zdroj: | Journal of cellular physiology. 233(9) |
| ISSN: | 1097-4652 |
| Popis: | LncRNA HOX transcript antisense intergenic RNA (HOTAIR) has been shown to play prominent roles in tumorigenesis. However, its precise molecular mechanism in glioma has not been completely elucidated. In this study, we found that HOTAIR was aberrantly up-regulated in glioma tissues and was negatively correlated with miR-126-5p expression. Next, we determined that HOTAIR promote glioma progression by sponging miR-126-5p. Subsequently, glutaminase (GLS) was confirmed to be a direct target of miR-126-5p using bioinformatics software and a luciferase reporter assay. Moreover, HOTAIR could modulate GLS expression by functioning as a competing endogenous RNA (ceRNA) for miR-126-5p. Taken together, our study clarified that the HOTAIR/miR-126/GLS pathway is involved in glioma progression and may potentially serve as a target for glioma therapy. |
| Databáze: | OpenAIRE |
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