Kcnb1 plays a role in development of the inner ear
| Autor: | Vladimir Korzh, Eugene V. Gasanov, Justyna Jędrychowska |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
Biology
Mechanotransduction Cellular 03 medical and health sciences 0302 clinical medicine Loss of Function Mutation Otolith formation otorhinolaryngologic diseases medicine Animals Inner ear Cilia Molecular Biology Zebrafish 030304 developmental biology Cell Proliferation 0303 health sciences Cell growth Cilium Cell Biology Voltage-gated potassium channel Kinocilium Zebrafish Proteins biology.organism_classification Cell biology Protein Transport medicine.anatomical_structure Potassium Channels Voltage-Gated Ear Inner sense organs Otic vesicle 030217 neurology & neurosurgery Developmental Biology |
| Zdroj: | Developmental biology. 471 |
| ISSN: | 1095-564X |
| Popis: | The function of the inner ear depends on the maintenance of high concentrations of K+ ions. The slow-inactivating delayed rectifier Kv2.1/KCNB1 channel works in the inner ear in mammals. The kcnb1 gene is expressed in the otic vesicle of developing zebrafish, suggesting its role in development of the inner ear. In the present study, we found that a Kcnb1 loss-of-function mutation affected development of the inner ear at multiple levels, including otic vesicle expansion, otolith formation, and the proliferation and differentiation of mechanosensory cells. This resulted in defects of kinocilia and stereocilia and abnormal function of the inner ear detected by behavioral assays. The quantitative transcriptional analysis of 75 genes demonstrated that the kcnb1 mutation affected the transcription of genes that are involved in K+ metabolism, cell proliferation, cilia development, and intracellular protein trafficking. These results demonstrate a role for Kv2.1/Kcnb1 channels in development of the inner ear in zebrafish. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect