Proteomic and Post-Translational Modification Profiling of Exosome-Mimetic Nanovesicles Compared to Exosomes
| Autor: | Andrew F. Hill, Lesley Cheng, Mitch Shambrook, Amirmohammad Nasiri Kenari, Allan Stensballe, David W. Greening, Kenneth Kastaniegaard |
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| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
Proteomics
Endosome therapeutic exosomes exosomes Exosomes Biochemistry Exosome 03 medical and health sciences proteomics Microscopy Electron Transmission Biomimetics Animals Humans mimetic-nanovesicles Molecular Biology 030304 developmental biology 0303 health sciences Chemistry Proteomic Profiling 030302 biochemistry & molecular biology Microvesicles Cell biology post-translational modification artificial extracellular vesicles Proteome Nanocarriers extracellular vesicles Protein Processing Post-Translational Ultracentrifugation Biogenesis |
| Zdroj: | Kenari, A N, Kastaniegaard, K, Greening, D W, Shambrook, M, Stensballe, A, Cheng, L & Hill, A F 2019, ' Proteomic and Post-Translational Modification Profiling of Exosome-Mimetic Nanovesicles Compared to Exosomes ', Proteomics, vol. 19, no. 8, 1800161 . https://doi.org/10.1002/pmic.201800161 |
| DOI: | 10.1002/pmic.201800161 |
| Popis: | Issues associated with upscaling exosome production for therapeutic use may be overcome through utilizing artificial exosomes. Cell-derived mimetic nanovesicles (M-NVs) are a potentially promising alternative to exosomes for clinical applicability, demonstrating higher yield without incumbent production and isolation issues. Although several studies have shown that M-NVs have similar morphology, size and therapeutic potential compared to exosomes, comprehensive characterization and to what extent M-NVs components mimic exosomes remain elusive. M-NVs were generated through the extrusion of cells and proteomic profiling demonstrated an enrichment of proteins associated with membrane and cytosolic components. The proteomic data herein reveal a subset of proteins that are highly abundant in M-NVs in comparison to exosomes. M-NVs contain proteins that largely represent the parental cell proteome, whereas the profile of exosomal proteins highlight their endosomally derived origin. This advantage of M-NVs alleviates the necessity of endosomal sorting of endogenous therapeutic proteins or RNA into exosomes. This study also highlights differences in protein post-translational modifications among M-NVs, as distinct from exosomes. Overall this study provides key insights into defining the proteome composition of M-NVs as a distinct from exosomes, and the potential advantage of M-NVs as an alternative nanocarrier when spontaneous endosomal sorting of therapeutics are limited. |
| Databáze: | OpenAIRE |
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