Angiopeptin, a somatostatin-14 analogue, decreases adhesiveness of rat Leukocytes to unstimulated and IL-1β-activated rat heart endothelial cells
| Autor: | Kevin Dunsky, Marie L. Foegh, Dariusz Leszczynski, Yejun Zhao, Michael D. Josephs |
|---|---|
| Rok vydání: | 1995 |
| Předmět: |
Time Factors
Endothelium Lymphocyte Stimulation CD18 CD11a Biology Peptides Cyclic General Biochemistry Genetics and Molecular Biology Flow cytometry Cell Adhesion Leukocytes medicine Animals General Pharmacology Toxicology and Pharmaceutics Dose-Response Relationship Drug medicine.diagnostic_test Cardiovascular Agents Heart Rats Inbred Strains General Medicine Molecular biology Rats Cell biology Dose–response relationship Somatostatin medicine.anatomical_structure Oligopeptides Interleukin-1 |
| Zdroj: | Life Sciences. 57:PL217-PL223 |
| ISSN: | 0024-3205 |
| DOI: | 10.1016/0024-3205(95)02112-v |
| Popis: | We have previously demonstrated that somatostatin-14 and its octapeptide analogue, angiopeptin, decrease the ability of rat heart endothelial cells to bind leukocytes [Leszczynski, et al., Reg. Pept. 43 (1993) 131-140]. Here, we examined whether exposure of leukocytes to angiopeptin modifies their adhesiveness to the unstimulated and to IL-1 beta-activated endothelium. Monolayers of unstimulated endothelial cells bind 274 +/- 12 leukocytes/mm2. Exposure of leukocytes for 1, 4 and 24 hours to angiopeptin (1 microM) reduced significantly (p < 0.05) adhesion of leukocytes from 274 +/- 12 to 188 +/- 10, 185 +/- 8 and 172 +/- 3 cells/mm2, respectively. Stimulation of endothelial cells with Il-1 beta (100 U/ml) for 24 hours increased endothelial adhesiveness from 274 +/- 12 to 381 +/- 17 adhering leukocytes/mm2. Exposure of leukocytes for 1, 4 and 24 hours to angiopeptin (1 microM) reduced significantly (p < 0.05) binding of leukocytes to IL-1 beta-activated endothelium from 381 +/- 17 to 237 +/- 8, 254 +/- 11 and 248 +/- 13 cells/mm2, respectively. Angiopeptin had no effect on the expression of lymphocyte function-associated molecule-1 (LFA-1; CD11a/CD18) by leukocytes, as assessed by flow cytometry. This suggests that angiopeptin modulates adhesive properties of leukocytes by (1) altering the expression of other than LFA-1 adhesion molecule(s) and/or (2) modulating the affinity of adhesion molecule(s) expressed by leukocytes. In conclusion, our results demonstrate that angiopeptin reduces leukocyte adhesiveness to unstimulated and to IL-1 beta-activated endothelium. It suggests that angiopeptin may suppress immune response via modulation of the leukocyte-endothelial interaction. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect