The effects of RNA interference mediated VEGF gene silencing on biological behavior of renal cell carcinoma and transplanted renal tumor in nude mice
| Autor: | Juan Zhou, Zhihua Cheng, Yang Zhang, Meng Gu, Ming-xi Xu, Ke Zhang, Shuai Wang, Guang Chen, Xiwei Sun, Haijun Yao, Wang Zhong, Qi Wang, Yu-Bing Peng, Siqiao Sun |
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| Rok vydání: | 2015 |
| Předmět: |
0301 basic medicine
Vascular Endothelial Growth Factor A Cancer Research Angiogenesis Genetic enhancement Gene Expression Mice Nude Biology 03 medical and health sciences chemistry.chemical_compound Mice 0302 clinical medicine RNA interference Cell Movement Cell Line Tumor Gene expression Genetics Gene silencing Animals Humans Gene Silencing RNA Small Interfering Carcinoma Renal Cell Cell Proliferation Neovascularization Pathologic Cell growth Cell migration General Medicine Molecular biology Immunohistochemistry Xenograft Model Antitumor Assays Kidney Neoplasms Vascular endothelial growth factor Disease Models Animal 030104 developmental biology Oncology chemistry 030220 oncology & carcinogenesis Female RNA Interference |
| Zdroj: | Cancer biomarkers : section A of Disease markers. 16(1) |
| ISSN: | 1875-8592 |
| Popis: | Objective This study was to explore the effects of RNA interference mediated vascular endothelial growth factor (VEGF) gene silencing on biological behavior of renal cell carcinoma (RCC), transplanted renal tumor and angiogenesis in nude mice. Methods The specific siRNA sequence targeting VEGF were designed and synthesized to construct hVEGF-siRNA plasmid which was transfected into RCC 786-O cells. Reverse transcriptase-polymerase chain reaction (RT-PCR) was used for the detection of VEGF gene expression and western blot was adopted for the examination of VEGF protein expression. The 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay was used to detect cell growth as well as cell migration and invasion. The transplanted renal tumor models in nude mice were established, and the growth condition of nude mice, and VEGF protein expression in transplanted tumor slices and the microvessel density (MVD) were detected. Results The expression level of VEGF mRNA in VEGF-siRNA group was significant lower than that in the control group and negative group, suggesting that establishment of plasmid specifically inhibited the expression of VEGF gene The expression level of VEGF protein in VEGF-siRNA group was significant lower than that in the control group and negative group. VEGF gene silencing has the significant inhibition effects on proliferation, migration and invasion of RCC 786-O cells. The tumor weight, VEGF protein positive rate and MVD in VEGF-siRNA group were significant lower than those in negative group and blank group. Conclusion The VEGF gene silencing could inhibit the cell proliferation, migration and invasion of RCC 786-O cells; inhibition of VEGF protein expression could prevent transplanted RCC growth and tumor angiogenesis. |
| Databáze: | OpenAIRE |
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