Bacillus natronophilus sp. nov., an alkaliphilic bacterium isolated from a soda lake
| Autor: | Mauricio Langleib, Rodolfo Javier Menes, Eliana Valentina Machin, Andrés Iriarte |
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| Přispěvatelé: | Menes Rodrigo Javier, Universidad de la República (Uruguay). Facultad de Ciencias. Instituto de Química Biológica., Machín Olivera Eliana Valentina, Universidad de la República (Uruguay). Facultad de Medicina., Iriarte Andrés, Universidad de la República (Uruguay). Facultad de Medicina., Langleib de Souza Mauricio, Universidad de la República (Uruguay). Facultad de Medicina. |
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0106 biological sciences
0301 basic medicine Bacillus natronophilus sp. nov Moderately halophilic Cellulase 010603 evolutionary biology 01 natural sciences Microbiology 03 medical and health sciences chemistry.chemical_compound Alkaliphile Food science Soda lake Ecology Evolution Behavior and Systematics Bacillus (shape) biology General Medicine biology.organism_classification 16S ribosomal RNA Halophile 030104 developmental biology chemistry Bacillus sp Xylanase biology.protein Peptidoglycan Bacteria |
| Zdroj: | COLIBRI Universidad de la República instacron:Universidad de la República |
| Popis: | An alkaliphilic, moderately halophilic, heterotrophic, rod-shaped, spore-forming bacterium (M30T) was isolated from a sediment sample collected from a soda lake (Lake Magadi, Tanzania). Strain M30T was strictly aerobic, catalase-positive, oxidase-negative and non-motile. Growth occurred at 12–43 °C (optimum, 25–30 °C), at pH 8.0–12 (optimum, pH 9.5–10) and at salinities of 0.5–15 % (w/v) NaCl (optimum 5 %). It utilized various sugars and organic acids as sole carbon sources and was positive for amylase, cellulase, gelatinase, protease and xylanase activities. The cell-wall peptidoglycan contained meso-diaminopimelic acid and the polar lipids consisted of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, one unidentified lipid and one unidentified phospholipid. The DNA G+C content was 48.9 mol%. The predominant menaquinone was MK-7 and the major fatty acids (>10 %) comprised anteiso-C15 : 0, iso-C15 : 0, and anteiso-C17 : 0. Phylogenetic analysis based on 16S rRNA gene sequence affiliated M30T to the genus Bacillus and showed the highest similarities to Bacillus populi FJAT-45347T (96.4 %) and Bacillus aurantiacus K1-5T (96.2 %). Based on the data from the current polyphasic study, M30T represents a novel species of the genus Bacillus , for which the name Bacillus natronophilus sp. nov. is proposed. The type strain is M30T (=JCM 32118T=CGMCC 1.16739T=MCC 3010T). |
| Databáze: | OpenAIRE |
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