Abnormalities of crystallins in the lens of the CatFraser mouse
| Autor: | R.J.M. Gold, A.T. Garber, L. Stirk |
|---|---|
| Rok vydání: | 1983 |
| Předmět: |
Electrophoresis
genetic structures Genotype Mice Inbred Strains Anatomy Biology Dosage effect Molecular biology Crystallins eye diseases Sensory Systems Cataract law.invention Lens (optics) Molecular Weight Cellular and Molecular Neuroscience Ophthalmology Mice Crystallin law Lens Crystalline Chromatography Gel Animals sense organs |
| Zdroj: | Experimental eye research. 36(2) |
| ISSN: | 0014-4835 |
| Popis: | We have analysed, by column chromatography and two-dimensional electrophoresis, the soluble proteins present in the lenses of normal mice and of mice heterozygous (Cat/+) and homozygous (Cat/Cat) for the Cat Fraser mutation which causes a dominantly inherited cataract. In Cat/+ and Cat/Cat lenses, the γ-crystallins comprise a smaller fraction, and the α-crystallins a greater fraction, of the total crystallins present than found in normal lenses. These changes in composition involve all the subunits of each crystallin class and show a dosage effect, the change being greater in Cat/Cat than Cat/+ lenses. In both Cat/+ and Cat/Cat lenses, the β H -crystallin aggregate is lost and subunits are present which are not detectable in normal lenses. |
| Databáze: | OpenAIRE |
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