Evaluation of the Goldmann-Witmer coefficient in the immunological diagnosis of ocular toxocariasis
Autor: | Jian Ji, Ge Zhi Xu, Ying Wen Bi, Xin Huang, Wen Jun Cao, Min Zhou, Zhu Jian Wang |
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Rok vydání: | 2015 |
Předmět: |
Ocular toxocariasis
Adult Male genetic structures Adolescent Veterinary (miscellaneous) 030231 tropical medicine Enzyme-Linked Immunosorbent Assay Immunologic Tests Immunoglobulin E Aqueous Humor 03 medical and health sciences Immunological Diagnosis Young Adult 0302 clinical medicine Medicine Animals Humans Eye Infections Parasitic Child Retrospective Studies Toxocariasis biology business.industry Infant Toxocara canis Specific igg Middle Aged biology.organism_classification eye diseases Infectious Diseases Canis Insect Science Intraocular fluid Child Preschool Immunology 030221 ophthalmology & optometry biology.protein Larva Migrans Parasitology Female sense organs Antibody business |
Zdroj: | Acta tropica. 158 |
ISSN: | 1873-6254 |
Popis: | Ocular toxocariasis (OT) is a zoonotic parasitic infection mainly caused by the intraocular tissue invasion of second-stage Toxocara canis or Toxocara cati larva. Measuring specific anti-T. canis antibodies in the intraocular fluid (IF) can increase OT diagnosis accuracy using Goldmann-Witmer coefficient (GWC). However, there is no systemic evaluation of GWC application in the immunological diagnosis of OT. To assess GWC for the immunodiagnosis of ocular toxocariasis, paired IF and serum samples from 72 patients diagnosed with OT were retrospectively analyzed for specific anti-T. canis IgG by enzyme linked immunosorbent assay (ELISA). GWC values were calculated to determine intraocular specific IgG production. Clinical features and other laboratory data were recorded, and their correlations with GWC evaluated. Of the 72 OT patients, 60 (83.33%) showed intraocular specific IgG production confirmed by GWC, while intraocular nonspecific IgE production was found in 64/69 (92.75%) cases. No significant correlation was found among clinical features and IF specific IgG production. Values for peripheral blood samples were lower than those of intraocular fluid regarding OT screening, and vitreous humor samples showed increased local specific IgG and nonspecific IgE production compared with aqueous humor samples. Overall, our results indicate that GWC and intraocular IgE production have referential values in diagnosing ocular toxocariasis. |
Databáze: | OpenAIRE |
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