Development of CMV Antibody Tests and Their Clinical Evaluation
| Autor: | J. Scherders, C. Dagelinckx, A. Hooijmans, F. Wielaard, C. T. Smit-Siebinga, F. Welle |
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| Rok vydání: | 1986 |
| Předmět: |
medicine.drug_class
Population Cmv infections Cytomegalovirus Blood Donors Enzyme-Linked Immunosorbent Assay Antibodies Viral Monoclonal antibody Antigen Humans Medicine Seroconversion education Netherlands education.field_of_study CMV Antibody biology business.industry Age Factors Hematology General Medicine Virology Immunology biology.protein Reagent Kits Diagnostic Antibody business Clinical evaluation |
| Zdroj: | Vox Sanguinis. 51:31-34 |
| ISSN: | 1423-0410 0042-9007 |
| Popis: | An anti-CMV ELISA was developed in which monoclonal antibodies to immobilize the CMV antigens are used. The test was compared with the IHA test and there was 98.6% agreement. Using the CF test as a referee, sensitivity and specificity of the ELISA were calculated to be 98.6 and 99.0%, respectively. The seroconversion rate among Dutch donors was estimated from the age-related prevalence of anti-CMV antibodies to be about 0.4%. Specific anti-CMV IgM was searched for in a large donor population using an antibody-capture IgM ELISA; 1 of 600 donor sera was found positive. This makes it unlikely that screening for IgM anti-CMV will effectively prevent posttransfusion CMV infections. In order to meet the demand for simpler tests, a one-step ELISA was recently developed, based on the inhibition principle. Preliminary studies with this test indicate a high sensitivity and specificity. |
| Databáze: | OpenAIRE |
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