Perivascular Macrophages Limit Permeability
| Autor: | Esra Güç, Safiyyah Ziyad, Michele De Palma, Ryan D. Freshman, Mario Leonardo Squadrito, Julia J. Mack, Witold W. Kilarski, Huanhuan He, Caroline Baer, Carmen M. Warren, M. Luisa Iruela-Arispe, Austin I. McDonald, Melody A. Swartz |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Pathology Time Factors capillary permeability Nude Vascular permeability Cell Communication Cardiorespiratory Medicine and Haematology Inbred C57BL Transgenic Mice Cell Movement capillaries Mesentery Phosphorylation Cells Cultured Skin Cultured Dextrans Transfection Cadherins CD macrophages Phenotype medicine.anatomical_structure Peritoneum Cardiology and Cardiovascular Medicine Fluorescein-5-isothiocyanate Cell signaling Cell type medicine.medical_specialty Ovalbumin Cells Adipose tissue macrophages Clinical Sciences Mice Nude Mice Transgenic Biology Article Capillary Permeability 03 medical and health sciences Antigens CD Peritoneal medicine Animals Humans Antigens Rhodamines Cadherin Macrophages Endothelial Cells Coculture Techniques Capillaries Mice Inbred C57BL 030104 developmental biology Cardiovascular System & Hematology Macrophages Peritoneal Myeloid-derived Suppressor Cell |
| Zdroj: | He, H; Mack, JJ; Güç, E; Warren, CM; Squadrito, ML; Kilarski, WW; et al.(2016). Perivascular Macrophages Limit Permeability. Arteriosclerosis, Thrombosis, and Vascular Biology, 36(11), 2203-2212. doi: 10.1161/ATVBAHA.116.307592. UCLA: Retrieved from: http://www.escholarship.org/uc/item/2mq001hp Arteriosclerosis, thrombosis, and vascular biology, vol 36, iss 11 Arteriosclerosis thrombosis and vascular biology |
| ISSN: | 1524-4636 1079-5642 |
| Popis: | Objective— Perivascular cells, including pericytes, macrophages, smooth muscle cells, and other specialized cell types, like podocytes, participate in various aspects of vascular function. However, aside from the well-established roles of smooth muscle cells and pericytes, the contributions of other vascular-associated cells are poorly understood. Our goal was to ascertain the function of perivascular macrophages in adult tissues under nonpathological conditions. Approach and Results— We combined confocal microscopy, in vivo cell depletion, and in vitro assays to investigate the contribution of perivascular macrophages to vascular function. We found that resident perivascular macrophages are associated with capillaries at a frequency similar to that of pericytes. Macrophage depletion using either clodronate liposomes or antibodies unexpectedly resulted in hyperpermeability. This effect could be rescued when M2-like macrophages, but not M1-like macrophages or dendritic cells, were reconstituted in vivo, suggesting subtype-specific roles for macrophages in the regulation of vascular permeability. Furthermore, we found that permeability-promoting agents elicit motility and eventual dissociation of macrophages from the vasculature. Finally, in vitro assays showed that M2-like macrophages attenuate the phosphorylation of VE-cadherin upon exposure to permeability-promoting agents. Conclusions— This study points to a direct contribution of macrophages to vessel barrier integrity and provides evidence that heterotypic cell interactions with the endothelium, in addition to those of pericytes, control vascular permeability. |
| Databáze: | OpenAIRE |
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