Serological evidence of West Nile virus infection in the horse population of northern Serbia
| Autor: | René van den Hoven, Strahinja Medić, Tamaš Petrović, Diana Lupulović, Norbert Nowotny |
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| Rok vydání: | 2013 |
| Předmět: |
Veterinary medicine
viruses Population Enzyme-Linked Immunosorbent Assay Antibodies Viral Microbiology Plaque reduction neutralization test Neutralization Tests Seroepidemiologic Studies Virology Surveys and Questionnaires Seroprevalence Animals Horses education Neutralizing antibody Asymptomatic Infections education.field_of_study biology virus diseases Horse General Medicine nervous system diseases Titer Infectious Diseases biology.protein Parasitology Horse Diseases Antibody Serbia West Nile virus West Nile Fever |
| Zdroj: | Journal of infection in developing countries. 8(7) |
| ISSN: | 1972-2680 |
| Popis: | Introduction: This study was conducted to evaluate the seroprevalence of West Nile virus (WNV) in the horse population of northern Serbia. Furthermore, it aimed to provide insight and an updated overview on the circulation of this re-emerging pathogen in this part of southeastern Europe. At the time of manuscript preparation, no clinical cases of WNV infection in horses were reported in this area. Methodology: Between 2007 and 2011, a total of 252 equine serum samples were collected from seven different locations in northern Serbia. The presence of WNV-reactive IgG antibodies was examined by using commercial and in-house ELISAs. Selected ELISA-positive samples were re-tested by a WNV lineage 2 plaque reduction neutralization test 90% (PRNT-90). Results: In 28.6% of the 252 tested equine serum samples antibodies against WNV were detected. The results obtained with the in-house ELISA corresponded to the outcome of the commercial kit in 90% of the samples. All selected WNV antibody ELISA-positive samples were confirmed by PRNT-90 with neutralizing antibody titers of 1:23 to > 1:512. Conclusion: This study confirms the circulation of WNV in northern Serbia. No striking regional differences in seroprevalences were identified suggesting WNV circulation also in other parts of Serbia. Distances between wetlands or forests and stud farms do not appear to have an influence on WNV seroprevalence. |
| Databáze: | OpenAIRE |
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