A frameshift mutation destabilizes androgen receptor messenger RNA in the Tfm mouse
| Autor: | Kathie L. Olsen, Nancy J. Charest, Elizabeth M. Wilson, Frank S. French, Dennis B. Lubahn, Zhong Xun Zhou |
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| Rok vydání: | 1991 |
| Předmět: |
Transcription
Genetic Molecular Sequence Data Biology Molecular cloning Kidney Polymerase Chain Reaction Frameshift mutation chemistry.chemical_compound Mice Endocrinology Complementary DNA Sequence Homology Nucleic Acid Animals Amino Acid Sequence RNA Messenger Cycloheximide Frameshift Mutation Molecular Biology Peptide sequence Base Sequence Nucleic acid sequence Chromosome Mapping General Medicine Blotting Northern Molecular biology Androgen receptor genomic DNA chemistry Receptors Androgen DNA Polymorphism Restriction Fragment Length |
| Zdroj: | Molecular endocrinology (Baltimore, Md.). 5(4) |
| ISSN: | 0888-8809 |
| Popis: | A composite mouse androgen receptor DNA sequence was obtained by amplifying genomic DNA or cDNA using the polymerase chain reaction. The open reading frame was 2,697 basepairs, encoding a polypeptide of 899 amino acids (98,204 mol wt). Amino acid sequence comparisons indicated that the mouse androgen receptor (AR) is 97% homologous with rat AR and 83% with human AR. The amino acid sequences of the three receptors are identical within the DNA- and steroid-binding domains. Northern blot analysis revealed the predominant mouse AR mRNA to be 10 kilobases (kb). A 1.7-kb mRNA species was detected in mouse kidney using a cDNA probe containing only 5' untranslated AR sequence. Lack of hybridization with AR-coding sequence probes suggested that the 1.7-kb mRNA was not a truncated form of AR mRNA. Sequencing of genomic DNA isolated from testicular feminized (Tfm) mice revealed a single base deletion in the N-terminal domain, resulting in a frameshift mutation. Cycloheximide treatment caused a dramatic increase in AR mRNA in kidneys of Tfm mice, but not wild-type mice, suggesting that the Tfm mutation results in an unstable AR mRNA. |
| Databáze: | OpenAIRE |
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