Melaleuca alternifolia formulations in the treatment of experimental pythiosis

Autor: Júlia de Souza Silveira, Carolina Litchina Brasil, Caroline Quintana Braga, Ândrios da Silva Moreira, Helen Cabaldi Franz, Rosimeri Zamboni, Eliza Simone Sallis, Ana Paula Albano, Cristina Gomes Zambrano, Lara Colles Araujo, Cristiane de Bona da Silva, Luciana Pötter, Sônia de Avila Botton, Daniela Isabel Brayer Pereira
Rok vydání: 2022
Předmět:
Zdroj: Braz J Microbiol
ISSN: 1678-4405
1517-8382
Popis: Essential oils (EO) are aromatic compounds from the plant secondary metabolism. Melaleuca alternifolia EO is well known for its medicinal properties and promising use as an antimicrobial agent. Pythiosis is a difficult-to-treat and emerging disease caused by the oomycete Pythium insidiosum. This study evaluated a nanoemulsion formulation of M. alternifolia (NEMA) in topical and intralesional application to treat experimental pythiosis. Dermal toxicity tests were performed on M. alternifolia EO in Wistar rats. Pythiosis was reproduced in rabbits (n = 9) that were divided into groups: group 1 (control), cutaneous lesions with daily topical application of a non-ionizable gel-based formulation and intralesional application of sterile distilled water every 48 h; group 2 (topical formulation), lesions treated daily with topical application of a non-ionizable gel-based formulation containing 5 mg/ml of NEMA; and group 3 (intralesional formulation), lesions treated with NEMA at 5 mg/ml in aqueous solution applied intralesionally/48 h. The animals were treated for 45 days, and the subcutaneous lesion areas were measured every 5 days. M. alternifolia EO showed no dermal toxicity. The lesion areas treated with intralesional NEMA reduced at the end of treatment, differing from groups 1 and 2 (P < 0.05). In the topically treated group, the lesion areas did not differ from the control group, although the number of hyphae significantly reduced (P < 0.05). Under the experimental conditions of this study, the NEMA formulations presented a favorable safety profile. However, further studies are required to evaluate if this safety applies to higher concentrations of NEMA and to validate its use in clinical pythiosis. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1007/s42770-022-00720-6.
Databáze: OpenAIRE
Externí odkaz: