Brine Shrimp Lethality Test Active Constituents and New Highly Oxygenated Seco-prezizaane-Type Sesquiterpenes from Illicium merrillianum
| Autor: | Kousuke Nakade, Mamiko Kondo, Yoshiyasu Fukuyama, Chun-Shu Yang, Jian-Mei Huang |
|---|---|
| Rok vydání: | 2002 |
| Předmět: |
Magnetic Resonance Spectroscopy
Brine shrimp Fractionation Illicium Mass Spectrometry Lethal Dose 50 chemistry.chemical_compound Spectroscopy Fourier Transform Infrared Drug Discovery Animals Organic chemistry Spectral data biology Plant Extracts Absolute configuration General Chemistry General Medicine biology.organism_classification Oxygen chemistry Fruit Chromatography Gel Methanol Artemia Sesquiterpenes |
| Zdroj: | Chemical and Pharmaceutical Bulletin. 50:133-136 |
| ISSN: | 1347-5223 0009-2363 |
| DOI: | 10.1248/cpb.50.133 |
| Popis: | In the study of bioactive substances in Illicium plants, the methanol extract of I. merrillianum showed brine shrimp lethality test (BST) activity at 200 microg/ml. Bioassay-guided fractionation of the BST active fractions resulted in the isolation of 4-O-methyleudesm-11-en-4alpha-ol, eudesmol-11-en-4alpha-ol and (-)-hinokinin as potent BST active compounds. On the other hand, four new highly oxygenated seco-prezizaane-type sesquiterpenes, merrilliortholactone (1), 2alpha-hydroxycycloparvifloralone (2), 2alpha-hydroxycycloparviflorolide (3), and 2alpha-hydroxyanisatin (4) were isolated from the BST-inactive polar fractions. The structures of new compounds were elucidated by extensive analyses of spectral data. Furthermore, the absolute configuration of 3 was established by the modified Mosher's method. Compounds 1--4 showed neither BST activity at 100 microg/ml nor neurite outgrowth-promoting activity. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|