Long-Chain Polyisoprenoids Are Synthesized by AtCPT1 in Arabidopsis thaliana
| Autor: | Katarzyna Manko, Przemyslaw Surowiecki, Liliana Surmacz, Ewa Swiezewska, Agnieszka Onysk |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
dolichols
Mutant Pharmaceutical Science Analytical Chemistry lcsh:QD241-441 03 medical and health sciences chemistry.chemical_compound Dolichol lcsh:Organic chemistry Arabidopsis Drug Discovery polyisoprenoids Homomeric Arabidopsis thaliana polyprenols Physical and Theoretical Chemistry protein N-glycosylation 030304 developmental biology 0303 health sciences biology Endoplasmic reticulum 030302 biochemistry & molecular biology Organic Chemistry rer2Δ and srt1Δ Saccharomyces cerevisiae mutant biology.organism_classification Yeast cis-prenyltransferase Complementation Biochemistry chemistry Chemistry (miscellaneous) Molecular Medicine lipids (amino acids peptides and proteins) T-DNA insertion mutant |
| Zdroj: | Molecules Volume 24 Issue 15 Molecules, Vol 24, Iss 15, p 2789 (2019) |
| ISSN: | 1420-3049 |
| DOI: | 10.3390/molecules24152789 |
| Popis: | Arabidopsis roots accumulate a complex mixture of dolichols composed of three families, (i.e., short-, medium- and long-chain dolichols), but until now none of the cis-prenyltransferases (CPTs) predicted in the Arabidopsis genome has been considered responsible for their synthesis. In this report, using homo- and heterologous (yeast and tobacco) models, we have characterized the AtCPT1 gene (At2g23410) which encodes a CPT responsible for the formation of long-chain dolichols, Dol-18 to -23, with Dol-21 dominating, in Arabidopsis. The content of these dolichols was significantly reduced in AtCPT1 T-DNA insertion mutant lines and highly increased in AtCPT1-overexpressing plants. Similar to the majority of eukaryotic CPTs, AtCPT1 is localized to the endoplasmic reticulum (ER). Functional complementation tests using yeast rer2&Delta or srt1&Delta mutants devoid of medium- or long-chain dolichols, respectively, confirmed that this enzyme synthesizes long-chain dolichols, although the dolichol chains thus formed are somewhat shorter than those synthesized in planta. Moreover, AtCPT1 acts as a homomeric CPT and does not need LEW1 for its activity. AtCPT1 is the first plant CPT producing long-chain polyisoprenoids that does not form a complex with the NgBR/NUS1 homologue. |
| Databáze: | OpenAIRE |
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