Maytenus ilicifolia dry extract protects normal cells, induces apoptosis and regulates Bcl-2 in human cancer cells
| Autor: | Luiz Alberto Lira Soares, Jonas Bispo Pessoa, Pedro Ros Petrovick, Raimundo Fernandes de Araújo Júnior, Ana Luiza C. de S. L. Oliveira, Vinícios Barreto Garcia, Gerlane Coelho Bernardo Guerra, Aurigena Antunes de Araújo, Tatiane Pereira de Souza |
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| Rok vydání: | 2013 |
| Předmět: |
Keratinocytes
Programmed cell death Down-Regulation Apoptosis General Biochemistry Genetics and Molecular Biology Flow cytometry Western blot medicine Humans Cytotoxic T cell medicine.diagnostic_test biology Caspase 3 Plant Extracts Maytenus Hep G2 Cells biology.organism_classification Antineoplastic Agents Phytogenic Molecular biology Gene Expression Regulation Neoplastic HaCaT Proto-Oncogene Proteins c-bcl-2 Immunology Gastritis medicine.symptom HT29 Cells |
| Zdroj: | Experimental Biology and Medicine. 238:1251-1258 |
| ISSN: | 1535-3699 1535-3702 |
| DOI: | 10.1177/1535370213494563 |
| Popis: | Maytenus is the largest genus of the family Celastraceae and the species Maytenus ilicifolia (popularly known as ‘Espinheira Santa’). It is widely used in traditional Brazilian medicine to treat stomach conditions including nausea, gastritis, and ulcers. In this study, the apoptotic effects of a spray-dried extract of M. ilicifolia (SDEMI) was evaluated using human hepatocellular cells (HepG2), colorectal carcinoma cells (HT-29), and normal keratinocytes (HaCaT). Cells were treated with SDEMI for 4 and 24 h, then were assayed for levels of apoptosis, caspase-3, and Bcl-2 by flow cytometry, immunostaining, and Western blot, respectively. Significant differences between groups were determined using analysis of variance ( P |
| Databáze: | OpenAIRE |
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