B cell receptor repertoire kinetics after SARS-CoV-2 infection and vaccination

Autor: Prasanti Kotagiri, Mark R. Wills, Kelvin Hunter, William Rae, Laura Bergamaschi, Ravindra K. Gupta, John Bradley, Nicholas J Matheson, Eoin F. McKinney, Myra Hosmillo, Paul A. Lyons, Menna R. Clatworthy, Zewen Tuong, Lorinda Turner, Ian Goodfellow, Rachael Bashford-Rogers, Federica Mescia, Kenneth G. C. Smith, Federico Pehuen Pereyra Gerber, Christoph Hess
Jazyk: angličtina
Rok vydání: 2022
Předmět:
Resource
B cell receptor repertoire
History
medicine.medical_specialty
Polymers and Plastics
B-Cell Receptor Repertoire
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)
education
Immunoglobulin Variable Region
Receptors
Antigen
B-Cell
Severity of Illness Index
Industrial and Manufacturing Engineering
General Biochemistry
Genetics and Molecular Biology
Clonal Evolution
Informed consent
Receptors
medicine
Immunoglobulin
Humans
Business and International Management
BNT162 Vaccine
B-Lymphocytes
business.industry
SARS-CoV-2
Repertoire
Vaccination
B-Cell
COVID-19
University hospital
SARS-CoV-2 vaccination
Immunoglobulin Heavy Chains
Immunoglobulin Isotypes
Kinetics
Somatic Hypermutation
Immunoglobulin
Spike Glycoprotein
Coronavirus
Somatic Hypermutation
Spike Glycoprotein
Coronavirus
Research centre
Family medicine
Antigen
Core laboratory
business
Zdroj: Cell Reports
Popis: B cells are important in immunity to both SARS-CoV-2 infection and vaccination, but B cell receptor (BCR) repertoire development in these contexts has not been compared. We analyse serial samples from 171 SARS-CoV-2-infected individuals and 63 vaccine recipients, and find the global BCR repertoire differs between them. Following infection, IgG1/3 and IgA1 BCRs increase, somatic hypermutation (SHM) decreases and, in severe disease, IgM and IgA clones are expanded. In contrast, after vaccination the proportion of IgD/M BCRs increase, SHM is unchanged and expansion of IgG clones is prominent. VH1-24, which targets the N-terminal domain (NTD) and contributes to neutralization, is expanded post-infection except in the most severe disease. Infection generates a broad distribution of SARS-CoV-2-specific clones predicted to target the spike protein, whilst a more focused response after vaccination mainly targets the spike’s receptor-binding domain. Thus the nature of SARS-CoV-2 exposure differentially impacts BCR repertoire development, potentially informing vaccine strategies.
Graphical Abstract
Kotagiri et al. find that SARS-CoV-2 infection versus vaccination induces distinct changes in the B cell receptor repertoire, including prominent clonal expansion in IgA and IgM after infection, but IgG after vaccination. A broad anti-spike response to infection contrasts with a narrower RBD-focused one after vaccination, potentially informing vaccination strategies.
Databáze: OpenAIRE
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