Renal expression of sigma 1 receptors in diabetic rats
| Autor: | Marija Vitlov Uljević, Ivana Milardović, Natalija Filipović, Katarina Vukojević, Sandra Kostic |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
sigma 1 receptors
diabetic nephropathy postnatal kidney maturation distal tubuli streptozotocin 0301 basic medicine Male medicine.medical_specialty Histology medicine.medical_treatment Intraperitoneal injection Kidney Proinflammatory cytokine Diabetes Mellitus Experimental Pathogenesis Diabetic nephropathy Rats Sprague-Dawley 03 medical and health sciences 0302 clinical medicine Internal medicine Diabetes mellitus medicine Animals Receptors sigma Diabetic Nephropathies Receptor business.industry Cell Biology General Medicine Streptozotocin medicine.disease Immunohistochemistry Rats 030104 developmental biology medicine.anatomical_structure Endocrinology 030220 oncology & carcinogenesis sense organs business medicine.drug Signal Transduction |
| Zdroj: | Acta histochemica. 122(6) |
| ISSN: | 1618-0372 |
| Popis: | The purpose of this study was to determine the changes in the expression of sigma 1 receptors (σ1Rs) in the kidney of diabetic rats, which could indicate their possible role in the pathogenesis of diabetic nephropathy (DN). Sprague-Dawley rats were were given intraperitoneal injection of 55 mg/kg streptozotocin (STZ) in order to induce type I of diabetes (DM1). Control and diabetic rats were sacrificed 2 weeks or 2 months after DM1 induction. Expression of σ1Rs was determined in kidneys of the experimental rats, using immunohistochemistry. The most prominent expression of σ1Rs was found in distal tubuli (DT). Results have shown significant increase in renal σ1Rs section percentage area of rats 2 months after DM1 induction, compared to both control group at the same age and diabetic group 2 weeks after induction (P < 0.01 both). Similarly, a number of immunoreactive DT increased in diabetic group 2 months after induction, compared to DM1 group 2 weeks after induction (P < 0.001). We also found a decrease of a number of immunoreactive DT 2 weeks post DM1 induction (P < 0.01). However, the same was found during maturation of the control rats (P < 0.001). In addition, a strong co-expression of σ1R and proinflammatory factor TGFβ was seen in vacuolated DT. The results indicate to the potential role of σ1Rs in postnatal maturation of the rat kidneys and in distal tubular damage in the pathogenesis of the diabetic nephropathy. We conclude that σ1Rs could be potential target in treatment of the diabetic nephropathy. |
| Databáze: | OpenAIRE |
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