A novel MHC-II targeted BVDV subunit vaccine induces a neutralizing immunological response in guinea pigs and cattle
| Autor: | Josefina Baztarrica, Lucía Rocha, Andrea Pecora, A. Wigdorovitz, Mario Acosta, Demian Bellido, José M. Escribano, Viviana Parreño |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
viruses
Protein subunit Guinea Pigs Cattle Diseases TARGETED VACCINE Culling Biology FIELD TRIAL CATTLE Antibodies Viral Virus Antigen Animals Neutralizing antibody Diarrhea Viruses Bovine Viral General Veterinary General Immunology and Microbiology Viral Vaccines General Medicine Virology Vaccination BOVINE VIRAL DIARRHOEA VIRUS Vaccines Subunit biology.protein SUBUNIT VACCINE Bovine Virus Diarrhea-Mucosal Disease Cattle Antibody purl.org/becyt/ford/4.3 [https] purl.org/becyt/ford/4 [https] Major histocompatibility |
| Zdroj: | CONICET Digital (CONICET) Consejo Nacional de Investigaciones Científicas y Técnicas instacron:CONICET |
| DOI: | 10.1111/tbed.13952 |
| Popis: | Bovine viral diarrhoea virus (BVDV) is a major cause of economic loss in the cattle industry, worldwide. Infection results in reduced productive performance, growth retardation, reduced milk production and increased susceptibility to other diseases leading to early culling of animals. There are two primary methods used to control the spread of BVDV: the elimination of persistently infected (PI) animals and vaccination. Currently, modified live or inactivated vaccines are used in BVDV vaccination programmes, but there are safety risks or insufficient protection, respectively, with these vaccines. Here, we report the development and efficacy of the first targeted subunit vaccine against BVDV. The core of the vaccine is the fusion of the BVDV structural protein, E2, to a single-chain antibody, APCH, together termed, APCH-E2. The APCH antibody targets the E2 antigen to the major histocompatibility type II molecule (MHC-II) present on antigen-presenting cells. Industrial production of the vaccine is carried out using the baculovirus expression vector system (BEVS) using single-use manufacturing technologies. This new subunit vaccine induces strong BVDV-specific neutralizing antibodies in guinea pigs and cattle. Importantly, in cattle with low levels of natural BVDV-specific neutralizing antibodies, the vaccine induced strong neutralizing antibody levels to above the protective threshold, as determined by a competition ELISA. The APCH-E2 vaccine induced a rapid and sustained neutralizing antibody response compared with a conventional vaccine in cattle. Fil: Bellido, Demian. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Baztarrica, Josefina. No especifíca; Fil: Rocha, Lucía. Instituto Nacional de Tecnología Agropecuaria; Argentina Fil: Pecora, Andrea. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación En Ciencias Veterinarias y Agronómicas. Instituto de Virología E Innovaciones Tecnológicas. - Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Pque. Centenario. Instituto de Virología E Innovaciones Tecnológicas; Argentina Fil: Acosta, Mario. No especifíca; Fil: Escribano, José M.. No especifíca; Fil: Parreño, Gladys Viviana. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación En Ciencias Veterinarias y Agronómicas. Instituto de Virología E Innovaciones Tecnológicas. - Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Pque. Centenario. Instituto de Virología E Innovaciones Tecnológicas; Argentina Fil: Wigdorovitz, Andrés. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación En Ciencias Veterinarias y Agronómicas. Instituto de Virología E Innovaciones Tecnológicas. - Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Pque. Centenario. Instituto de Virología E Innovaciones Tecnológicas; Argentina |
| Databáze: | OpenAIRE |
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