Determination of hair ketamine cut-off value from Hong Kong ketamine users by LC-MS/MS analysis
| Autor: | Karen K.L. Ho, Ran Duan, Zack C.F. Wong, Stanley P.H. Ip, Ada W.S. Yip, Jenny S.C. Ng, Winki Y.Y. Ng, KY Zhu, K. Wing Leung, Janet Y.M. Ho, Karl Wah Keung Tsim |
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| Rok vydání: | 2015 |
| Předmět: |
Adult
Male Adolescent Population Street drugs Ketamine abuse 01 natural sciences Pathology and Forensic Medicine 03 medical and health sciences Young Adult 0302 clinical medicine Tandem Mass Spectrometry Norketamine Lc ms ms medicine Humans Ketamine 030216 legal & forensic medicine education education.field_of_study business.industry Illicit Drugs Cut off value 010401 analytical chemistry Chromatography liquid Reproducibility of Results 0104 chemical sciences Anesthesia Hong Kong Female business Law medicine.drug Chromatography Liquid Hair |
| Zdroj: | Forensic science international. 259 |
| ISSN: | 1872-6283 |
| Popis: | Ketamine is one of the most frequent abused drugs in Hong Kong and South-East Asia, and the cases of ketamine abused have been reported worldwide. Hair has been commonly used as a specimen for the proof of chronic drug abused because of its non-invasiveness and long detection windows. The determinations of ketamine in hair with varieties of state-of-the-art instruments and detection methods have been developed in the past decade; however, the cut-off value for ketamine abuser has not been developed according to the international guidelines. The aim of this study is to propose a cut-off value for ketamine in hair by analyzing ketamine and its metabolite norketamine by LC-MS/MS method in a population of ketamine users in Hong Kong. The limit of detection (LOD) and limit of quantification (LOQ) for ketamine and norketamine were 20pg/mg and 100pg/mg, respectively. From 977 ketamine abusers, the cut-off value for ketamine in hair was proposed to be 400pg/mg of hair. This proposed cut-off value is the concentration of hair ketamine when over 90% of samples are being detected with the presence of norketamine, which is a proof of ketamine abuse. This value could be applied as a screening or occupational cut-off for reference. |
| Databáze: | OpenAIRE |
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