Measurement of intrasynaptosomal free calcium by using the fluorescent indicator quin-2
| Autor: | R. M. Marchbanks, M J Brammer, R H Ashley |
|---|---|
| Rok vydání: | 1984 |
| Předmět: |
Potassium
Sodium Guinea Pigs Inorganic chemistry Ionophore chemistry.chemical_element Lithium Calcium Synaptic Transmission Biochemistry Cytosol medicine Animals Molecular Biology Fluorescent Dyes Calcium metabolism Chemistry Cell Membrane Depolarization Cell Biology Acetylcholine Spectrometry Fluorescence Aminoquinolines Biophysics Thermodynamics Research Article Synaptosomes medicine.drug |
| Zdroj: | Biochemical Journal. 219:149-158 |
| ISSN: | 1470-8728 0264-6021 |
| DOI: | 10.1042/bj2190149 |
| Popis: | The recently synthesized calcium indicator quin −2 was incorporated into synaptosomes from guinea-pig cerebral cortex following uptake and internal hydrolysis of quin −2 tetra-acetoxymethyl ester. Incubation in physiological media containing 1 mM- or 2 mM-CaCl2 led to equilibrium cytosolic ionized calcium concentrations of 85 +/- 10 nM and 205 +/- 5 nM respectively (mean +/- S.E.M. from eight and eighteen preparations respectively). Cytosolic Ca2+ was elevated following increases in external Ca2+ concentration, plasma membrane depolarization, mitochondrial inhibition, calcium ionophore addition or replacement of external sodium by lithium. Preliminary experiments were performed to assess changes in cytosolic Ca2+ accompanying the release of the neurotransmitter acetylcholine. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|